Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes

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Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes

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Publication Article, peer reviewed scientific
Title Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes
Author(s) Wang, Xiaoju ; Falk, Magnus ; Ortiz, Roberto ; Matsumura, Hirotoshi ; Bobacka, Johan ; Ludwig, Roland ; Bergelin, Mikael ; Gorton, Lo ; Shleev, Sergey
Date 2012
English abstract
We report on the fabrication and characterisation of a gold-nanoparticle (AuNP)-basedmediatorlesssugar/oxygen biofuel cell (BFC) operating in neutral sugar-containing buffers and human physiological fluids, such as blood and plasma. First, Corynascus thermophilus cellobiose dehydrogenase (CtCDH) and Myrothecium verrucaria bilirubin oxidase (MvBOx), used as anodic and cathodic bioelements, respectively, were immobilised on goldelectrodesmodified with 20 nm AuNPs. Detailed characterisation and optimisation of a new CDH/AuNP-based bioanode were performed and the following fundamental parameters were obtained: (i) the redox potential of the haem-containing centre of the enzyme was measured to be 75 mV vs. NHE, (ii) the surface coverage of CtCDH was found to be 0.65 pmol cm−2 corresponding to a sub-monolayer coverage of the thiol-modified AuNPs by the enzyme, (iii) a turnover number for CtCDH immobilised on thiol-modified AuNPs was calculated to be ca. 0.5 s−1, and (iv) the maximal current densities as high as 40 μA cm−2 were registered in sugar-containing neutral buffers. Second, both biomodified electrodes, namely the CtCDH/AuNP-based bioanode and the MvBOx/AuNP-based biocathode, were combined into a functional BFC and the designed biodevices were carefully investigated. The following characteristics of the mediator-, separator- and membrane-less, miniature BFC were obtained: in phosphate buffer; an open-circuit voltage of 0.68 V, a maximum power density of 15 μW cm−2 at a cell voltage of 0.52 V and in human blood; an open-circuit voltage of 0.65 V, a maximum power density of 3 μW cm−2 at a cell voltage of 0.45 V, respectively. The estimated half-lives of the biodevices were found to be >12, <8, and <2 h in a sugar-containing buffer, human plasma, and blood, respectively. The basic characteristics of mediatorlesssugar/oxygen BFCs were significantly improved compared with previously designed biodevices, because of the usage of three-dimensional AuNP-modifiedelectrodes.
DOI (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Biosensors and Bioelectronics;1
Volume 31
ISSN 0956-5663
Pages 219-225
Language eng (iso)
Subject(s) enzymatic fuel cells
direct electron transfer
gold nanoparticles
cellobiose dehydrogenase
bilirubin oxidase
Research Subject Categories::NATURAL SCIENCES
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