Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes

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Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes

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dc.contributor.author Wang, Xiaoju
dc.contributor.author Falk, Magnus
dc.contributor.author Ortiz, Roberto
dc.contributor.author Matsumura, Hirotoshi
dc.contributor.author Bobacka, Johan
dc.contributor.author Ludwig, Roland
dc.contributor.author Bergelin, Mikael
dc.contributor.author Gorton, Lo
dc.contributor.author Shleev, Sergey
dc.date.accessioned 2012-04-20T07:06:40Z
dc.date.available 2012-04-20T07:06:40Z
dc.date.issued 2012 en_US
dc.identifier.citation 219-225 en_US
dc.identifier.issn 0956-5663 en_US
dc.identifier.uri http://hdl.handle.net/2043/13677
dc.description.abstract We report on the fabrication and characterisation of a gold-nanoparticle (AuNP)-basedmediatorlesssugar/oxygen biofuel cell (BFC) operating in neutral sugar-containing buffers and human physiological fluids, such as blood and plasma. First, Corynascus thermophilus cellobiose dehydrogenase (CtCDH) and Myrothecium verrucaria bilirubin oxidase (MvBOx), used as anodic and cathodic bioelements, respectively, were immobilised on goldelectrodesmodified with 20 nm AuNPs. Detailed characterisation and optimisation of a new CDH/AuNP-based bioanode were performed and the following fundamental parameters were obtained: (i) the redox potential of the haem-containing centre of the enzyme was measured to be 75 mV vs. NHE, (ii) the surface coverage of CtCDH was found to be 0.65 pmol cm−2 corresponding to a sub-monolayer coverage of the thiol-modified AuNPs by the enzyme, (iii) a turnover number for CtCDH immobilised on thiol-modified AuNPs was calculated to be ca. 0.5 s−1, and (iv) the maximal current densities as high as 40 μA cm−2 were registered in sugar-containing neutral buffers. Second, both biomodified electrodes, namely the CtCDH/AuNP-based bioanode and the MvBOx/AuNP-based biocathode, were combined into a functional BFC and the designed biodevices were carefully investigated. The following characteristics of the mediator-, separator- and membrane-less, miniature BFC were obtained: in phosphate buffer; an open-circuit voltage of 0.68 V, a maximum power density of 15 μW cm−2 at a cell voltage of 0.52 V and in human blood; an open-circuit voltage of 0.65 V, a maximum power density of 3 μW cm−2 at a cell voltage of 0.45 V, respectively. The estimated half-lives of the biodevices were found to be >12, <8, and <2 h in a sugar-containing buffer, human plasma, and blood, respectively. The basic characteristics of mediatorlesssugar/oxygen BFCs were significantly improved compared with previously designed biodevices, because of the usage of three-dimensional AuNP-modifiedelectrodes. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.subject enzymatic fuel cells en_US
dc.subject direct electron transfer en_US
dc.subject gold nanoparticles en_US
dc.subject cellobiose dehydrogenase en_US
dc.subject bilirubin oxidase en_US
dc.subject.classification Sciences en_US
dc.title Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Health and Society en
dc.identifier.doi http://dx.doi.org/10.1016/j.bios.2011.10.020 en_US
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES en_US
dc.relation.ispartofpublication Biosensors and Bioelectronics;1
dc.relation.ispartofpublicationvolume 31 en_US
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