Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces - effects of surface characteristics

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Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces - effects of surface characteristics

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dc.contributor.author Svendsen, Ida
dc.contributor.author Santos, Olga
dc.contributor.author Sotres, Javier
dc.contributor.author Wennerberg, Ann
dc.contributor.author Breding, Karin
dc.contributor.author Arnebrant, Thomas
dc.contributor.author Lindh, Liselott
dc.date.accessioned 2012-10-24T10:11:34Z
dc.date.available 2012-10-24T10:11:34Z
dc.date.issued 2012 en_US
dc.identifier.citation 87-97 en_US
dc.identifier.issn 1029-2454 en_US
dc.identifier.uri http://hdl.handle.net/2043/14264
dc.description.abstract Ellipsometry and mechanically assisted sodium dodecyl sulphate elution was utilized to study the adsorption of human serum albumin (HSA), human immunoglobulin G (IgG), and laminin-1, as well as competitive adsorption from a mixture of these proteins on spin-coated and sintered hydroxyapatite (HA) surfaces, respectively. The HA surfaces were characterized with respect to wettability and roughness by means of water contact angles and atomic force microscopy, respectively. Both surface types were hydrophilic, and the average roughness (Sa) and surface enlargement (Sdr) were lower for the sintered compared to the spin-coated HA surfaces. The adsorbed amounts on the sintered HA increased as follows: HSA < laminin-1 < IgG < the protein mixture. For the competitive adsorption experiments, the adsorbed fractions increased accordingly: HSA < laminin-1 < IgG on both types of HA substratum. However, a higher relative amount of HSA and laminin-1 and a lower relative amount of IgG was found on the spin-coated surfaces compared to the sintered surfaces. The effects observed could be ascribed to differences in surface roughness and chemical composition between the two types of HA substratum, and could have an influence on selection of future implant surface coatings. en_US
dc.language.iso eng en_US
dc.publisher Taylor & Francis Group en_US
dc.subject.classification Medicine en_US
dc.title Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces - effects of surface characteristics en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi http://dx.doi.org/10.1080/08927014.2011.653562 en_US
dc.subject.srsc Research Subject Categories::ODONTOLOGY en_US
dc.relation.ispartofpublication Biofouling : The Journal of Bioadhesion and Biofilm Research;1
dc.relation.ispartofpublicationvolume 28 en_US
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