Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified au electrodes

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Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified au electrodes

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Publication Article, peer reviewed scientific
Title Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified au electrodes
Author(s) Vaz-Dominguez, Cristina ; Pita, Marcos ; de Lacey, Antonio ; Shleev, Sergey ; Cuesta, Angel
Date 2012
English abstract
The successive steps of laccase immobilization on chem. modified Au electrodes were monitored using ATR-SEIRAS and in situ STM. Successful covalent immobilization of the enzyme on Au electrodes modified by a mixed aminophenyl-mercaptohexanol adlayer and on Au electrodes modified by a 4-aminothiophenyl SAM via a Schiff base reaction followed by the formation of amide bonds is revealed by the emergence of the corresponding bands in the ATR-SEIRA spectra, and an enzyme coverage on aminophenyl-mercaptohexanol-modified Au electrodes of about (7.27 ± 1.93) × 1011 laccase units per cm2 was calcd. from STM images. The small differences between the ATR-SEIRA spectra of the enzyme immobilized on aminophenyl-mercaptohexanol-modified Au electrodes and the ATR-SEIRA spectra of the enzyme immobilized on 4-aminothiophenyl-modified Au electrodes are attributed to a different orientation of the immobilized enzyme due to the presence on the surface of aminophenyl-mercaptohexanol-modified Au electrodes of OH functional groups that favor an orientation of laccase with the Cu T1 center of the enzyme facing the electrode surface, thus, allowing a high activity for direct electrocatalysis of the ORR at low overpotentials.
DOI http://dx.doi.org/10.1021/jp303818p (link to publisher's fulltext)
Publisher American Chemical Society
Host/Issue Journal of Physical Chemistry C;31
Volume 116
ISSN 1932-7447
Pages 16532-16540
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/14301 (link to this page)

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