Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified au electrodes

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Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified au electrodes

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dc.contributor.author Vaz-Dominguez, Cristina
dc.contributor.author Pita, Marcos
dc.contributor.author de Lacey, Antonio
dc.contributor.author Shleev, Sergey
dc.contributor.author Cuesta, Angel
dc.date.accessioned 2012-11-08T08:35:55Z
dc.date.available 2012-11-08T08:35:55Z
dc.date.issued 2012 en_US
dc.identifier.citation 16532-16540 en_US
dc.identifier.issn 1932-7447 en_US
dc.identifier.uri http://hdl.handle.net/2043/14301
dc.description.abstract The successive steps of laccase immobilization on chem. modified Au electrodes were monitored using ATR-SEIRAS and in situ STM. Successful covalent immobilization of the enzyme on Au electrodes modified by a mixed aminophenyl-mercaptohexanol adlayer and on Au electrodes modified by a 4-aminothiophenyl SAM via a Schiff base reaction followed by the formation of amide bonds is revealed by the emergence of the corresponding bands in the ATR-SEIRA spectra, and an enzyme coverage on aminophenyl-mercaptohexanol-modified Au electrodes of about (7.27 ± 1.93) × 1011 laccase units per cm2 was calcd. from STM images. The small differences between the ATR-SEIRA spectra of the enzyme immobilized on aminophenyl-mercaptohexanol-modified Au electrodes and the ATR-SEIRA spectra of the enzyme immobilized on 4-aminothiophenyl-modified Au electrodes are attributed to a different orientation of the immobilized enzyme due to the presence on the surface of aminophenyl-mercaptohexanol-modified Au electrodes of OH functional groups that favor an orientation of laccase with the Cu T1 center of the enzyme facing the electrode surface, thus, allowing a high activity for direct electrocatalysis of the ORR at low overpotentials. en_US
dc.language.iso eng en_US
dc.publisher American Chemical Society en_US
dc.subject.classification Sciences en_US
dc.title Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified au electrodes en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Health and Society en
dc.identifier.doi http://dx.doi.org/10.1021/jp303818p en_US
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES en_US
dc.relation.ispartofpublication Journal of Physical Chemistry C;31
dc.relation.ispartofpublicationvolume 116 en_US
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