Laminin coating promotes calcium phosphate precipitation on titanium discs in vitro

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Laminin coating promotes calcium phosphate precipitation on titanium discs in vitro

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Publication Article, peer reviewed scientific
Title Laminin coating promotes calcium phosphate precipitation on titanium discs in vitro
Author(s) Bougas, Kostas ; Franke Stenport, Victoria ; Tengvall, Pentti ; Currie, Fredrik ; Wennerberg, Ann
Date 2011
English abstract
Objectives: The objective of this study was to investigate the effect of a laminin coating on calcium phosphate precipitation on three potentially bioactive titanium surfaces in simulated body fluid. Material and Methods: Blasted titanium discs were prepared by alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA) and subsequently coated with laminin. A laminin coated blasted surface (B) served as a positive control while a blasted non coated (B-) served as a negative control. Surface morphology was examined by Scanning Electron Microscopy (SEM). The analysis of the precipitated calcium and phosphorous was performed by Energy Dispersive X-ray Spectroscopy (EDX). Results: The thickness of the laminin coating was estimated at 26 Å by ellipsometry. Interferometry revealed that the coating process did not affect any of the tested topographical parameters on μm level when comparing B to B-. After 2 weeks of incubation in SBF, the alkali-heat treated discs displayed the highest calcium phosphate deposition and the B group showed higher levels of calcium phosphate than the B- group. Conclusions: Our results suggest that laminin may have the potential to be used as a coating agent in order to enhance the osseoinductive performance of biomaterial surfaces, with the protein molecules possibly functioning as nucleation centres for apatite formation. Nevertheless, in vivo studies are required in order to clarify the longevity of the coating and its performance in the complex biological environment.
DOI http://dx.doi.org/10.5037/jomr.2011.2405 (link to publisher's fulltext)
Publisher Stilus Optimus
Host/Issue Journal of Oral and Maxillofacial Research;4
Volume 2
ISSN 2029-283X
Pages 10
Language eng (iso)
Subject(s) laminin
titanium
biomaterials
calcium phosphates
dental implants
osseointegration
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/14454 (link to this page)

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