Utskrift från Malmö högskola - mah.se
Browsing Articles /OD by Issue Date
MUEP
JavaScript is disabled for your browser. Some features of this site may not work without it.
Browsing Articles /OD by Issue Date
Now showing items 1-20 of 664
-
Chemical and mechanical dental caries removing system Carisolv Ericson, Dan
Journal article in Shikai tenbo;3 Dental outlook;3 (1999)peer-reviewed 
scientific
ARTICLE -
Transient reduction of mutans streptococci on tooth surfaces using a ... Hoszek, Anders; Ericson, Dan
Journal article in Swedish dental journal;2-3 (1999)peer-reviewed
scientific
ARTICLE
English abstract: Chlorhexidine (CHX) has been incorporated in polymer-based varnishes to reduce mutans streptococci (ms) by the sustained release of CHX. Such varnishes often adhere well to teeth initially but are easily peeled off. To be effective, repeated application is necessary. Glass ionomer (GI) cements interacts with tooth minerals to form a stronger bond, and the inherent brittleness of the cement makes it difficult to remove in large fragments. The fluoride content may also reduce demineralisation. The aim of this study was to observe whether ms could be reduced interproximally and in saliva by a single application of a GI cement containing 3.3% CHX gluconate (CHX-GI). After professional tooth cleaning and a mouth rinse for 2 min with 0.2% CHX, the teeth of six subjects were coated with CHX-GI cement. An additional six subjects were treated with a GI cement that did not contain CHX, and seven subjects received professional tooth cleaning only. Ms samples were taken interproximally with the tooth pick method before and after treatment. Interproximal levels were classified according to the number of colony-forming units (c.f.u.) found: 0, 1-20, 21-100, and > 100. Saliva ms were sampled with the Strip Mutans method. After four weeks, the interproximal levels of ms had decreased only in the CHX-GI group (p < 0.05). In this group 9 of 14 highly colonised sites (> 100 c.f.u.) remained reduced throughout this period. In the GI and the untreated group a slight increase of ms interproximally was seen after one week. The interproximal ms scores in all groups approached baseline levels after 8 weeks. There were no significant differences in saliva ms levels between the groups during the test period. GI cement may be a possible vehicle for CHX in reducing ms interproximally. -
Concentration dependence of adsorption from human whole resting saliv... Lindh, Liselott; Arnebrant, Thomas; Isberg, Per-Erik; Glantz, Per-Olof
Journal article in Biofouling;3 (1999)peer-reviewed
scientific
ARTICLE
English abstract: The rates and plateau values of adsorption at the solid/ liquid interface from human whole resting saliva from three male donors were studied in vitro. The influence of saliva concentration, the total protein content and the wettability of the exposed solids was investigated. Ellipsometry was used to study the rate and plateau values of the adsorption process in situ. The solid surfaces used were hydrophilic, negatively charged, silica and hydrophobic methylated silica. Salivary films were formed by adsorption from solutions of 0.05%, 0.10%, 0.20%, 0.50%, 1.0% and 2.0% (v/v) saliva in phosphate buffered saline. The results revealed that adsorption onto both types of surfaces was a rapid and, as expected, a concentration dependent process. The adsorbed amounts were in the range of monomolecular layers and were found to be larger onto the hydrophobic than the hydrophilic surfaces (p≤0.0062). A strong concentration dependence was denoted in all cases (p< 0.0001). Inter-individual differences were found not to be significant (0.0636 ≤ p≤ 0.8508) at any of the saliva concentrations. However, an interaction was shown (p < 0.0001) between the protein concentrations and the donors, indicating qualitative differences in protein content and surface activity. -
Macromolecular organization of saliva: identification of ‘insoluble’ ... Wickström, Claes; Christersson, Cecilia; Davies, Julia; Carlstedt, Ingemar
Journal article in Biochemical Journal (2000)peer-reviewed
scientific
ARTICLE
English abstract: Stimulated human submandibular’sublingual (HSMSL) and whole saliva were separated into sol and gel phases and mucins were isolated by density-gradient centrifugation in CsCl/4M guanidinium chloride. MUC5B and MUC7 were identified using anti-peptide antisera raised against sequences within the MUC5B and MUC7 apoproteins respectively. MUC7 was found mainly in the sol phase of both HSMSL and whole saliva, but some MUC7 was consistently present in the gel phase, suggesting that this mucin may interact with the salivary gel matrix. In HSMSL saliva, MUC5B was found in the gel phase ; however, most of the material was ‘ insoluble ’ in guanidinium chloride and was only brought into solution by reduction. In whole saliva, the MUC5B mucin was present both in the sol and gel phases although some material was again ‘ insoluble ’. Rate-zonal centrifugation of whole saliva showed that MUC5B mucins in the sol phase were smaller than those in the gel phase, suggesting differences in oligomerization and/or degradation. Antibodies against IgA, secretory component, lysozyme and lactoferrin were used to study the distribution of non-gel-forming proteins in the different phases of saliva. The majority of these proteins was found in the sol phase of both HSMSL and whole saliva. However, a significant fraction was present in the gel phase of whole saliva, suggesting a post-secretory interaction with the salivary gel matrix. A monoclonal antibody against a parotid salivary agglutinin was used to show that this protein is present mainly in the gel phase of both whole saliva and parotid secretion. -
Caries risk assessment: a comparison between the computer program 'Ca... Bratthall, Douglas; Hänsel Petersson, Gunnel
Journal article in Swedish dental journal;4 (2000)peer-reviewed
scientific
ARTICLE
English abstract: The 'Cariogram', which is an interactive PC-program for caries risk evaluation, illustrates the interaction between caries related factors and expresses the caries risk graphically. It also demonstrates a weighted impact of the different etiological factors on the risk. The aim of this paper was to use the Cariogram program on a set of patients, and to compare the outcome of its risk evaluation with those made by dental hygienists and dentists. A questionnaire, containing the descriptions of five patients with detailed information on nine factors generally associated with caries, was given to the participants. They were asked to rank the patients according to their 'chance to avoid dental caries' during the coming year. The results were compared with the assessments obtained from the Cariogram. Results: 73.5% of the dental hygienists and 78.5% of the dentists ranked the patients for caries risk either identically or with only one deviation when compared to the Cariogram. It was concluded that the 'opinion' of the Cariogram on caries risk was in agreement with that of the majority of the participants. In addition, the Cariogram program induced discussions about the relative impact of etiological factors of caries. It is envisaged that the Cariogram can serve as one further tool in the teaching of caries risk. -
Film-forming properties and viscosities of saliva substitutes and hum... Christersson, Cecilia; Lindh, Liselott; Arnebrant, Thomas
Journal article in European Journal of Oral Sciences;5 (2000)peer-reviewed
scientific
ARTICLE
English abstract: Hypo-salivation, related to medical remedies, is an increasing clinical problem. Studies report a weak correlation between subjective mouth dryness and objective sialometry. This indicates that both quantity and quality of saliva are important for the surface-associated functions of saliva, such as lubrication and hydration, to be expressed. Film-forming properties and viscosities of three saliva substitutes were compared to human saliva. Adsorption to surfaces was measured by ellipsometry, infrared spectroscopy and drop-volume technique. Viscosity measurements were carried out using an oscillating rheometer. Saliva, with the lowest viscosity value and the highest protein content, presented superior film retention on both hydrophilic and hydrophobic surfaces. The carboxymethylcellulose-based MAS 84 showed intermediate values of viscosity, poorest ability to reduce surface tension, and negligible film-forming capacity. The porcine mucin-based Saliva Orthana showed about twice the viscosity of saliva and film-forming capability on preferably hydrophobic substrates. Salinum, a linseed extract, possessed the highest viscosity value and an initial surface tension close to that of saliva. The film retention on hydrophilic surfaces was not as effective as for saliva. The results indicate that the film-forming capacity of saliva substitutes is a property also to be considered in the exploration of clinically effective artificial salivas. -
N-terminal cleavage of the salivary MUC5B mucin. Analogy with the Van... Wickström, Claes; Carlstedt, Ingemar
Journal article in Journal of Biological Chemistry;50 (2001)peer-reviewed
scientific
ARTICLE
English abstract: Sequence similarities between the oligomeric mucins (MUC2, MUC5AC, MUC5B) and the von Willebrand factor suggest that they may be assembled in a similar way. After oligomerization, a fragment corresponding to the D1 and D2 domains is released from the von Willebrand factor. This cleavage does not appear to occur in pig submaxillary mucin, the only mammalian mucin in which this cleavage has been examined thus far, but whether other oligomeric mucins undergo N terminus proteolysis is not known. Antibodies recognizing the D1, D2, D3, and the first Cys domains in MUC5B were established and used to investigate to what extent proteolytic cleavage occurs within the N-terminal part of salivary MUC5B. The antibodies against the D1 and D2 domains identified a polypeptide corresponding in size to a MUC5B fragment generated by cleavage within the D' domain analogously with the von Willebrand factor propolypeptide. The antibodies did not recognize the main mucin population, suggesting that the major part of salivary MUC5B is subjected to this cleavage. An antibody recognizing the D3 domain was used to reveal a second cleavage site in the "soluble" but not in the "insoluble" MUC5B fraction: the first structural difference observed between soluble and insoluble salivary MUC5B. The identification of these cleavage events shows that the N-terminal sites for MUC5B oligomerization are present in the D3 domain and/or in domains located C-terminal to this part of the molecule. -
Identification of a nonmucin glycoprotein (gp-340) from a purified re... Thornton, David; Davies, Julia; Kirkham, Sara; Gautrey, Alex; Khan, Nagma; Richardson, Paul; Sheehan, John
Journal article in Glycobiology;11 : Oxford Journals (2001)peer-reviewed
scientific
ARTICLE
English abstract: Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins. -
Distribution of iodine 125-labeled alpha1-microglobulin in rats after... Larsson, Jörgen; Wingårdh, Karin; Berggård, Tord; Davies, Julia; Lögdberg, Lennart; Strand, Sven-Erik; Åkerström, Bo
Journal article in Jouranl of Laboratory and Clinical Medicine;3 : Academic Press (2001)peer-reviewed
scientific
ARTICLE
English abstract: The 28-kd plasma protein α1-microglobulin is found in the blood of mammals and fish in a free, monomeric form and as high-molecular-weight complexes with molecular masses above 200 kd. In this study, iodine 125–labeled free and high-molecular weight rat α1-microglobulin (a mixture of α1-microglobulin/α1-inhibitor-3 and α1-microglobulin/fibronectin complexes) were injected intravenously into rats. The distribution of the proteins was measured by using scintillation camera imaging. Both forms of 125I-labeled α1-microglobulin were rapidly cleared from the blood, with a half-life of 2 and 16 minutes for the initial and late phase, respectively, for free α1-microglobulin; and a half-life of 3 and 130 minutes for the initial and late phase, respectively, for the complexes. After 45 minutes, 6%, 16%, 27%, 13%, and 34% of the free 125I-labeled α1-microglobulin and 18%, 21%, 6%, 10%, and 42% of the 125I-labeled α1-microglobulin complexes were found in the blood, gastrointestinal tract, kidneys, liver, and the remainder of the body, respectively. The local distribution of injected 125I-labeled α1-microglobulin in intestines and kidneys was investigated by microscopy and autoradiography. In the intestine, both forms were distributed in the basal layers, villi, and luminal contents. The results also suggested intracellular labeling of epithelial cells. Well-defined local regions containing higher concentrations of injected protein could be seen in the intestine. In the kidneys, both forms were found mostly in the cortex. Free 125I-labeled α1-microglobulin was found predominantly in epithelial cells of a subset of the tubules, whereas the 125I-labeled complexes were more evenly distributed. Intracellular labeling was indicated for both α1-microglobulin forms. The results thus indicate a rapid transport of 125I-labeled α1-microglobulin from the blood to most tissues. (J Lab Clin Med 2001;137:165-75). -
Äldre och tandvård Zimmerman, Mikael; Ericson, Dan
Journal article in Tand & hälsoguiden (2001)ARTICLE -
HLA-DR4 and salivary immunoglobulin A reactions to oral streptococci Wallengren, Marie-Louise; Ericson, Dan; Hamberg, Kristina; Johnson, U
Journal article in Oral microbiology and immunology;1 (2001)peer-reviewed
scientific
ARTICLE
English abstract: The aim of this study was to describe and compare salivary immunoglobulin A (IgA) antibody reactions to extracts of strains of three oral streptococci in human leukocyte antigen (HLA)-DR4-positive and -DR4-negative subjects. Whole paraffin-stimulated saliva samples were collected from 27 apparently healthy subjects. Previous HLA typing showed that 20 subjects were DR4 positive and 7 were DR4 negative. HLA-DRB1*04 subtyping was performed among the DR4-positive subjects. Whole-cell antigen extracts from Streptococcus mutans (KPSK 2), Streptococcus sobrinus (OMZ 65) and Streptococcus parasanguis (Nt 62) were separated in SDS-PAGE. The antigens were immunoblotted with diluted saliva (Western blot), scanned and analyzed in a computer system. All immunoblot bands were recorded in DR4-positive and DR4-negative saliva pools, and bands with an optical density >or=0.1 were selected for analysis in individual salivas. The DR4-negative subjects in general had more immunoblot bands and more distinct bands than did the DR4-positive subjects. A higher concentration of total IgA in saliva was correlated with more bands, especially to antigens separated from S. mutans. When the number of bands was calculated per IgA unit, significant differences were observed between DR4-positive and DR4-negative salivas. This was particularly seen for S. mutans and S. parasanguis. As the number of bands was analyzed in relation to DR4 subgroups, DRB1*04, there was a lower salivary IgA activity to S. mutans in the DRB1*0401 and *0404. The variable level of correlation previously demonstrated for S. mutans colonisation and serologically defined DR4 positive subjects might be explained by the heterogeneity in this group, and the relation should be sought on a subgroup level. -
An in vitro study of initial adsorption from human parotid and subman... Lindh, Liselott; Glantz, Per-Olof; Isberg, Per-Erik; Arnebrant, Thomas
Journal article in Biofouling;3 : Taylor and Francis (2001)peer-reviewed
scientific
ARTICLE
English abstract: The influence of saliva concentration, saliva total protein content and the wetting characteristics of exposed solids on in vitro film formation was studied by the technique of in situ ellipsometry. The rates and plateau values of adsorption (45 min) at solid/liquid interfaces (hydrophilic silica and hydrophobic methylated silica surfaces) were determinated for human parotid (HPS) and submandibular/sublingual (HSMSLS) resting saliva solutions (0.1 and 1.0%, (v/v), saliva in phosphate buffered saline). Adsorption rates were related to a model assuming mass transport through an unstirred layer adjacent to the surface. The results showed that the adsorption was rapid, concentration dependent and higher on hydrophobic than on hydrophilic surfaces. Analysis of the influence of protein concentration on the adsorbed amounts demonstrated an interaction between protein concentration and the two surfaces for HPS and HSMSLS, respectively. This may indicate differences in binding mode. Inter-individual differences were found not to be significant at the 1% level of probability. Comparison of the observed adsorption and calculated diffusion rates suggest that on hydrophilic surfaces initial adsorption of proteins diffusing at rates corresponding to those of statherin and aPRPs takes place, whereas on hydrophobic surfaces lower molecular mass compounds appear to be involved. -
Gastric MUC5AC and MUC6 are large oligomeric mucins which differ in s... Nordman, Henrik; Davies, Julia; Lindell, Gert; De Bolos, Carmé; Real, Francisco; Carlstedt, Ingemar
Journal article in Biochemical Journal : The Biochemical Society (2002)peer-reviewed
scientific
ARTICLE
English abstract: Gastric MUC5AC and MUC6 mucins were studied using polyclonal antibodies. Immunohistochemistry showed MUC5AC to originate from the surface epithelium, whereas MUC6 was produced by the glands. Mucins from the surface epithelium or glands of corpus and antrum were purified using CsCl/4M guanidinium chloride density-gradient centrifugation. MUC5AC appeared as two distinct populations at 1.4 and 1.3 g/ml, whereas MUC6, which was enriched in the gland tissue, appeared at 1.45 g/ml. Reactivity with antibodies against the Le(b) structure (where Le represents the Lewis antigen) followed the MUC5AC distribution, whereas antibodies against the Le(y) structure and reactivity with the GlcNAc-selective Solanum tuberosum lectin coincided with MUC6, suggesting that the two mucins are glycosylated differently. Rate-zonal centrifugation of whole mucins and reduced subunits showed that both gastric MUC5AC and MUC6 are oligomeric glycoproteins composed of disulphide-bond linked subunits and that oligomeric MUC5AC was apparently smaller than MUC6. A heterogeneous population of 'low-density' MUC5AC mucins, which were smaller than the 'high-density' ones both before and after reduction, reacted with an antibody against a variable number tandem repeat sequence within MUC5AC, suggesting that they represent precursor forms of this mucin. Following ion-exchange HPLC, both MUC5AC and MUC6 appeared as several distinct populations, probably corresponding to 'glycoforms' of the mucins, the most highly charged of which were found in the gland tissue. -
Treatment Outcome of Headache After Occlusal Appliance Therapy in a R... Ekberg, EwaCarin; Vallon, Danila; Nilner, Maria
Journal article in Swedish Dental Journal;3 (2002)peer-reviewed
scientific
ARTICLE
English abstract: The aim of this study was to evaluate headaches before and after treatment with a stabilisation appliance and a control appliance in a randomised controlled trial in patients having temporomandibular disorders (TMD) of mainly arthrogenous origin. The effect of treatment was evaluated both in a short and in long-term perspective. 60 patients (mean age 30 years) with TMD of mainly arthrogenous origin were studied. The patients were selected from patients referred for treatment of TMD during a 3-year period to the Department of Stomathognathic Physiology, Faculty of Odontology, Malmo University. The 60 patients included in the study were randomly assigned to a treatment (T) or a control (C) group. The study was performed as a randomised controlled trial including evaluation of treatment effect on tension-type headache after 10 weeks, 6 and 12 months. At the 10 weeks follow-up, the patients who reported a negative treatment outcome and/or discomfort associated with the use of the appliances had their appliances readjusted. Patients who demanded further treatment received a stabilisation appliance (21 patients from the C-group), creating a new mixed (M) group. All the 60 patients reported frequency of headache from rarely up to daily at the start of the study. In the T-group 76% and in the C-group 83% of the patients reported headache at least once a week or more before treatment. At the 10 weeks follow-up, a statistically significant difference was found regarding headache several times a week or more between the T- and C-groups and within the T-group. At the 6 months follow-up, a statistically significant reduction was found in headache several times a week or more in the T- and M-groups. The number of patients with headache once a week or more decreased significantly in the T- and M-groups at the 12 months follow-up. We conclude that the stabilisation appliance seems to have an effect on the frequency of tension-type headache both in a short and in a long-term perspective in patients with TMD of mainly arthrogenous origin. -
Long-term Follow-up of Intra-articular Injections into the Tem-poroma... Vallon, Danila; Åkerman, Sigvard; Nilner, Maria; Petersson, Arne
Journal article in Swedish dental journal;4 (2002)peer-reviewed
scientific
ARTICLE
English abstract: A long-term (12 years) follow-up of treatment with intra-articular in-jections into the temporomandibular joint (TMJ) of steroid or non-steroid agents was performed in 21 patients with rheumatoid arthritis (RA) and symptomatic TMJs. The aim of the study was to compare symptoms, signs and radiological appearance of the TMJ initially and at the follow-up in this group of patients. Eleven patients were as-signed to a steroid group and 10 patients to a non-steroid group. Ini-tial and follow-up clinical and radiological examination procedures were the same. The radiological evaluation was based on a grading system using standard reference films. At follow-up, 14 patients re-ported no pain from the TMJ and positive changes in most clinical variables were found in both groups. Radiographic follow-up exami-nation was performed on 12 patients. Initially, all but 4 of the 24 joints had structural bone changes. At follow-up, 2 joints had lower, 11 joints had unchanged and 11 joints had higher radiological grades. Two out of 5 and 3 out of 10 joints in the steroid and non-steroid group, respectively, showed progression of structural bone changes. Among 9 untreated joints, 6 had higher radiological grades and 3 were unchanged. In the 11 TMJs with higher radiological grades at follow-up, there was in most cases moderate progression of erosive changes. The results suggest that the long-term development of symptoms and signs from the TMJ in patients previously treated was good and the long-term progression of joint destruction was low for both steroid and non-steroid agents in this patient group with RA. -
Evaluation of a computer program for caries risk assessment in school... Hänsel Petersson, Gunnel; Twetman, Svante; Bratthall, Douglas
Journal article in Cariesies Research;5 (2002)peer-reviewed
scientific
ARTICLE
English abstract: The 'Cariogram' is an interactive PC program for caries risk evaluation. It takes into account the interactions between caries-related factors and expresses a graphic assessment of the risk. The aim of this study was to assess the caries risk in schoolchildren using the Cariogram and to evaluate the program by comparing the caries risk assessments with the actual change in DMF. A 2-year prospective study on 446 schoolchildren, 10-11 years old, was conducted. At baseline, data on general health, diet, oral hygiene and use of fluoride were obtained. Saliva analyses included mutans streptococci and lactobacilli counts, buffer capacity and secretion rate. DMFT and DMFS were calculated from records and bitewing radiographs. Scores were entered and caries risk was assessed. Re-examination for caries was done after 2 years. The children were divided into 5 groups according to the assessed caries risk at baseline. Where the Cariogram predicted a 0-20% (high risk), 21-40%, 41-60%, 61-80% and 81-100% (low risk) chance of avoiding new lesions, 8, 35, 42, 73 and 83%, respectively, had no new lesions 2 years later. Logistic regression analyses were carried out. When the Cariogram was included, only two factors, the Cariogram (p < 0.001) and the DMFS at baseline, i.e. past caries experience (p = 0.001), turned out to be significantly associated with caries increment. The Cariogram was the most powerful explanatory variable. When the Cariogram was excluded, lactobacillus count, mutans streptococci, diet intake frequency and DMFS at baseline were significantly associated with caries increment. The Cariogram predicted caries increment more accurately than any included single-factor model. How this finding can be translated into daily practice in the best and most practical way is a matter for future research. -
Respiratory tract mucins: structure and expression patterns Davies, Julia; Herrman, Annkatrin; Russell, Wayne; Svitacheva, Naila; Wickström, Claes; Carlstedt, Ingemar
Journal article in Novartis Foundation symposium (2002)scientific
ARTICLE
English abstract: Goblet cells produce mainly MUC5AC, but also MUC5B and some MUC2 in apparently ‘irritated’ airways. MUC5B dominates in the submucosal glands although a little MUC5AC and MUC7 are usually present. MUC4 originates from the ciliated cells. After separation into a gel and a sol phase, lysozyme and lactoferrin are enriched in the salivary gel phase suggesting that mucus may act as a matrix for ‘protective’ proteins on the mucosal surface. A salivary MUC5B N-terminal fragment consistent with a cleavage event in the D’ domain was de-tected with antibodies against various N-terminal peptide sequences suggesting that assembly of MUC5B occurs through a mechanism similar to that of the von Willebrand factor. Identification of additional cleavage sites C-terminal to the D’ domain suggests that most of the N-terminal low-glycosylated part of MUC5B may be removed without affecting the oligomeric nature of the mucin. Possibly, the generation of mucins with different macromolecular properties through proteolytic ‘processing’ is one way of adapting the mucus polymer matrix to meet local physiological demands. Monomeric mucins that appear to turn over rapidly in the airway epithelium have been identified using radiolabelled mucin precursors. ‘Shedding’ of such mucins after microbe attachment may prevent colonization of epithelial surfaces. -
Adsorption of MUC5B and the role of mucins in early salivary film for... Lindh, Liselott; Glantz, Per-Olof; Carlstedt, Ingemar; Wickström, Claes; Arnebrant, Thomas
Journal article in Colloids and Surfaces B: Biointerfaces;2 : Elsevier (2002)peer-reviewed
scientific
ARTICLE
English abstract: Salivary mucins are known to play important roles in the formation of oral salivary films. The aims of the present study were to investigate the behaviour of salivary mucins at solid surfaces with different wettabilities, as well as the influence of electrolyte on the adsorption behaviour. A pure preparation of human salivary MUC5B was used together with a commercial one of bovine submaxillary mucin (BSM). Amounts adsorbed from freshly prepared solutions onto hydrophilic and hydrophobic surfaces versus time were measured in situ by ellipsometry. At low concentrations, larger amounts were adsorbed onto hydrophobic than onto hydrophilic silica indicating a higher affinity for the former surfaces. Furthermore, on hydrophilic surfaces adsorbed amounts of MUC5B and BSM show good agreement at low concentrations (<0.10 mgml^-^1). However, at higher concentrations MUC5B adsorbed to a lower extent than BSM. At hydrophobic surfaces, isotherm shapes were similar for the two preparations, but the amounts were shifted to higher values for MUC5B. Finally, the presence of electrolyte increased adsorption and the increase was more pronounced on hydrophilic surfaces. The increased adsorption at a higher ionic strength indicates a more compact structure of the mucin due to electrostatic screening and the fact that the effect was more pronounced on the hydrophilic surfaces points to a higher relative importance of electrostatic interactions in this case. We conclude that the two mucins investigated behave in a qualitatively similar manner and show the highest affinity for hydrophobic surfaces. -
On the adsorption of human proline-rich proteins (PRP-1 and PRP-3) an... Lindh, Liselott; Glantz, Per-Olof; Strömberg, Nicklas; Arnebrant, Thomas
Journal article in Biofouling;2 (2002)peer-reviewed
scientific
ARTICLE
English abstract: The objective of the present study was to investigate the adsorption of PRP-1, PRP-3 and statherin to solid surfaces in terms of dependence on concentration, the presence of electrolyte and surface wettability. Time resolved in situ ellipsometry was used to determine the adsorbed amounts and adsorption rates of pure PRP-1, PRP-3 and statherin onto pure (hydrophilic) and methylated (hydrophobized) silica surfaces. The initial film build-up was fast and plateaus were reached within 10 min at all concentrations for both types of surfaces and all proteins. The observed adsorption and calculated diffusion rates of PRP-1, PRP-3 and statherin, respectively, indicated that the initial adsorption was mass transport controlled at low concentrations. At hydrophobic surfaces, isotherm shapes and adsorbed amounts were similar for PRP-1 and PRP-3, while statherin adsorbed to a higher extent. At hydrophilic surfaces only PRP-1 adsorbed substantially, while for PRP-3 and statherin adsorbed amounts were low. The presence of Ca 2+ ions in the phosphate buffer solution increased the adsorption of statherin and PRP-3 on hydrophobic surfaces, while PRP-1 was unaffected. On hydrophilic surfaces, all three proteins adsorbed in higher amounts in NaCl, compared to CaCl 2 at similar ionic strength. It is concluded that acidic PRPs (PRP-1 and PRP-3) and statherin readily form films on a variety of materials and solution conditions, showing that their functions may be fulfilled under a wide range of conditions. -
A 6- and 12-month follow-up of appliance therapy in TMD patients: a f... Ekberg, EwaCarin; Nilner, Maria
Journal article in International journal of prosthodontics;6 (2002)peer-reviewed
scientific
ARTICLE
English abstract: PURPOSE: This study compared the long-term effects of treatment with a stabilization appliance and treatment with a control appliance in patients with temporomandibular disorders (TMD). MATERIALS AND METHODS: In a controlled trial, 60 TMD patients with tem-poromandibular joint (TMJ) pain were evaluated after 10 weeks of treatment with either a stabilization appliance or a control appliance. At the 10-week follow-up, the 60 patients were assigned to one of three groups according to their demand for treatment. Group T, the treatment group, comprised 30 patients treated with a stabilization appliance; group C, the control group, comprised nine patients treated with a control appliance; and group M, the mixed treatment group, comprised 21 patients treated with first a control appliance and then a stabilization appliance. Signs and symptoms were evaluated in all three groups at 6- and 12-month follow-ups. RESULTS: At the 6- and 12-month follow-ups, a significant reduction in TMJ pain as measured on a visual analogue scale was found in all three groups, and a significant decrease in signs and symptoms was found in groups T and M. CONCLUSION: After 6 and 12 months of use, the stabilization appli-ance was found to still be effective in the alleviation of signs and symptoms in patients with TMD. Many patients in group C changed to a stabilization appliance at the 1 0-week follow-up, which significantly reduced the number of patients in this group. Most patients reported positive change in overall subjective symptoms in this trial. The stabilization appliance can therefore be recommended for patients with TMD.
Now showing items 1-20 of 664