Browsing Articles /HS by Title

Activity based assessment (BIA) – inter-rater reliability and staff e...
Kjellin, Lars; Cizinsky Sjödahl, Rosmarie; Eklund, Mona Journal article in Scandinavian Journal of Occupational Therapy (2008)	peer-reviewed scientific ARTICLE

Adsorption behaviour of statherin and a statherin peptide onto hydrox...
Santos, Olga; Liselott, Lindh; Kosoric, Jelena; Hector, Mark; Anderson, Paul Journal article in Journal of Interface and Colloid Science 2 : Elsevier (2007)	peer-reviewed scientific ARTICLE

alpha1-antitrypsin inhibits Moraxella catarrhalis MID protein-induced...
Gjörloff Wingren, Anette; Hadzic, Radinka; Nita, Izabela; Tassidis, Helena; Riesbeck, Kristian; Janciauskiene, Sabina Journal article in Immunology Letters;2 : Elsevier (2006)	peer-reviewed scientific ARTICLE

English abstract:

alpha1-antitrypsin (AAT) is a major circulating and tissues inhibitor of serine proteinases implicated in the regulation of inflammation and host defence. There is now increasing evidence that AAT may also exhibit anti-inflammatory activities independent of its protease inhibitor function. This study was undertaken to investigate the effects of native (inhibitory) and polymerized (non-inhibitory) forms of AAT on MID (Moraxella IgD binding protein)-induced human tonsillar B cell activation in vitro. We found that 0.5 ug/ml MID induces B cell proliferation and stimulates IL-6 release (p<0.001) relative to non-stimulated controls. Both native and polymerized AAT (0.5 mg/ml) inhibited MID-stimulated B cell proliferation in a similar manner (by 70%, p<0.001), whereas MID-induced IL-6 release was more strongly suppressed by polymerized (9.9-fold, p<0.001) as compared to native AAT (2.8-fold, p<0.01). Electrophoretic analysis of cell culture media did not indicate any interaction between AAT and MID, and flow cytometry data showed no competition for the same receptor. The effects of AATs were observed whether added together with MID or 2 h after MID-addition to cell cultures. Thus, our data demonstrate that AAT inhibits MID-induced B cell activation in vitro that is unrelated to its protease inhibitory activity and is not dependent on MID binding to the cell surface.α1-Antitrypsin (AAT) is a major circulating and tissues inhibitor of serine proteinases implicated in the regulation of inflammation and host defence. There is now increasing evidence that AAT may also exhibit anti-inflammatory activities independent of its protease inhibitor function. This study was undertaken to investigate the effects of native (inhibitory) and polymerized (non-inhibitory) forms of AAT on MID (Moraxella IgD binding protein)-induced human tonsillar B cell activation in vitro. We found that 0.5 μg/ml MID induces B cell proliferation and stimulates IL-6 release (p < 0.001) relative to non-stimulated controls. Both native and polymerized AAT (0.5 mg/ml) inhibited MID-stimulated B cell proliferation in a similar manner (by 70%, p < 0.001), whereas MID-induced IL-6 release was more strongly suppressed by polymerized (9.9-fold, p < 0.001) as compared to native AAT (2.8-fold, p < 0.01). Electrophoretic analysis of cell culture media did not indicate any interaction between AAT and MID, and flow cytometry data showed no competition for the same receptor. The effects of AATs were observed whether added together with MID or 2 h after MID-addition to cell cultures. Thus, our data demonstrate that AAT inhibits MID-induced B cell activation in vitro that is unrelated to its protease inhibitory activity and is not dependent on MID binding to the cell surface.

Hadzic et al.pdf (235.5Kb)

Browsing Articles /HS by Title

MUEP

Browsing Articles /HS by Title

Search

Browse

All contents

This Collection

My Account

Adsorption from Black Tea and Red Wine onto IN Vitro Salivary Pellicl...
Joiner, Andrew; Arnebrant, Thomas; Malmsten, M; Elofsson, U.M. Journal article in European journal of oral sciences;5 : Blackwell Publisher (2003)	peer-reviewed scientific ARTICLE

Adsorption of chlorhexidine and black tea onto in vitro salivary pell...
Arnebrant, Thomas; Joiner, Andrew; Elofsson, Ulla Journal article in European Journal of Oral Sciences (2006)	peer-reviewed scientific ARTICLE

Adsorption of delmopinol at the solid/liquid interface - the role of ...
Svensson, Olof; Halthur, Tobias; Sjödin, Torgny; Arnebrant, Thomas Journal article in Journal of Colloid and Interface Science;1 : Elsevier Inc. (2010)	peer-reviewed scientific ARTICLE

Adsorption of HSA, IgG and laminin-1 on model titania surfaces – effe...
Santos, Olga; Svendsen, Ida E; Lindh, Liselott; Arnebrant, Thomas Journal article in Biofouling;9 : Taylor & Francis (2011)	peer-reviewed scientific ARTICLE