HLA-DR4 and salivary immunoglobulin A reactions to oral streptococci

DSpace Repository

HLA-DR4 and salivary immunoglobulin A reactions to oral streptococci

Show full item record

Files for download

Find Full text There are no files associated with this item.


Simple item record

Publication Article, peer reviewed scientific
Title HLA-DR4 and salivary immunoglobulin A reactions to oral streptococci
Author(s) Wallengren, Marie-Louise ; Ericson, Dan ; Hamberg, Kristina ; Johnson, U
Date 2001
English abstract
The aim of this study was to describe and compare salivary immunoglobulin A (IgA) antibody reactions to extracts of strains of three oral streptococci in human leukocyte antigen (HLA)-DR4-positive and -DR4-negative subjects. Whole paraffin-stimulated saliva samples were collected from 27 apparently healthy subjects. Previous HLA typing showed that 20 subjects were DR4 positive and 7 were DR4 negative. HLA-DRB1*04 subtyping was performed among the DR4-positive subjects. Whole-cell antigen extracts from Streptococcus mutans (KPSK 2), Streptococcus sobrinus (OMZ 65) and Streptococcus parasanguis (Nt 62) were separated in SDS-PAGE. The antigens were immunoblotted with diluted saliva (Western blot), scanned and analyzed in a computer system. All immunoblot bands were recorded in DR4-positive and DR4-negative saliva pools, and bands with an optical density >or=0.1 were selected for analysis in individual salivas. The DR4-negative subjects in general had more immunoblot bands and more distinct bands than did the DR4-positive subjects. A higher concentration of total IgA in saliva was correlated with more bands, especially to antigens separated from S. mutans. When the number of bands was calculated per IgA unit, significant differences were observed between DR4-positive and DR4-negative salivas. This was particularly seen for S. mutans and S. parasanguis. As the number of bands was analyzed in relation to DR4 subgroups, DRB1*04, there was a lower salivary IgA activity to S. mutans in the DRB1*0401 and *0404. The variable level of correlation previously demonstrated for S. mutans colonisation and serologically defined DR4 positive subjects might be explained by the heterogeneity in this group, and the relation should be sought on a subgroup level.
DOI http://dx.doi.org/10.1034/j.1399-302x.2001.160108.x (link to publisher's fulltext)
Host/Issue Oral microbiology and immunology;1
Volume 16
ISSN 0902-0055
Pages 45-53
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/10688 (link to this page)

This item appears in the following Collection(s)

Show full item record



My Account