Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin

DSpace Repository

Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin

Details

Files for download

Find Full text There are no files associated with this item.

Overview of item record
Publication Article, peer reviewed scientific
Title Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin
Author Thornton, David ; Davies, Julia ; Kirkham, Sara ; Gautrey, Alex ; Khan, Nagma ; Richardson, Paul ; Sheehan, John
Date 2001
English abstract
Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.
DOI http://dx.doi.org/10.1093/glycob/11.11.969 (link to publisher's fulltext)
Publisher Oxford Journals
Host/Issue Glycobiology;11
Volume 11
ISSN 0959-6658
Pages 969-977
Language eng (iso)
Subject Gp340
Mucus
MUC5B
MUC5AC
Medicine
Research Subject Categories::MEDICINE::Chemistry::Biochemistry
Research Subject Categories::MEDICINE::Morphology, cell biology, pathology::Cell biology::Medical cell biology
Handle http://hdl.handle.net/2043/11105 Permalink to this page
Facebook

This item appears in the following Collection(s)

Details

Search


Browse

My Account

Statistics