Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin

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Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin

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Publication Article, peer reviewed scientific
Title Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin
Author(s) Thornton, David ; Davies, Julia ; Kirkham, Sara ; Gautrey, Alex ; Khan, Nagma ; Richardson, Paul ; Sheehan, John
Date 2001
English abstract
Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.
DOI http://dx.doi.org/10.1093/glycob/11.11.969 (link to publisher's fulltext)
Publisher Oxford Journals
Host/Issue Glycobiology;11
Volume 11
ISSN 0959-6658
Pages 969-977
Language eng (iso)
Subject(s) Gp340
Mucus
MUC5B
MUC5AC
Medicine
Research Subject Categories::MEDICINE::Chemistry::Biochemistry
Research Subject Categories::MEDICINE::Morphology, cell biology, pathology::Cell biology::Medical cell biology
Handle http://hdl.handle.net/2043/11105 (link to this page)

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