Differential effects of Pseudomonas aeruginosa on biofilm formation by different strains of Staphylococcus epidermidis.

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Differential effects of Pseudomonas aeruginosa on biofilm formation by different strains of Staphylococcus epidermidis.

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Publication Article, peer reviewed scientific
Title Differential effects of Pseudomonas aeruginosa on biofilm formation by different strains of Staphylococcus epidermidis.
Author(s) Pihl, Maria ; Davies, Julia ; Chávez de Paz, Luis Eduardo ; Svensäter, Gunnel
Date 2010
English abstract
Pseudomonas aeruginosa and Staphylococcus epidermidis are common opportunistic pathogens associated with medical device-related biofilm infections. 16S rRNA fluorescence in situ hybridization and confocal scanning laser microscopy were used to study these two bacteria in dual-species biofilms. We have shown that fresh isolates of S. epidermidis form biofilms more avidly on polymer surfaces than laboratory strains. In dual-species biofilms the presence of P. aeruginosa reduced biofilm formation by S. epidermidis, although the different clinical isolates differed in their susceptibility to this effect. The most resistant fresh isolate coexisted with P. aeruginosa for up to 18 hours and was also resistant to the effects of culture supernatant from P. aeruginosa biofilms which caused dispersal of other S. epidermidis strains from established biofilms. Thus, different strains of S. epidermidis differed in their capacity to withstand the action of P. aeruginosa, with some fresh isolates being better equipped than laboratory strains to coexist in biofilms with P. aeruginosa. Our data suggest that where S. epidermidis and P. aeruginosa are present on abiotic surfaces such as medical devices, S. epidermidis biofilm-formation can be inhibited by P. aeruginosa through two mechanisms: disruption by extracellular products, possibly polysaccharides, and, in the later stages, by cell lysis.
DOI http://dx.doi.org/10.1111/j.1574-695X.2010.00697.x (link to publisher's fulltext)
Publisher Wiley Blackwell
Host/Issue FEMS Immunology and Medical Microbiology;3
Volume 46
ISSN 0928-8244
Pages 439-446
Language eng (iso)
Subject(s) dual-species biofilms
competition
strain differences
abiotic surfaces
Medicine
Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases
Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Microbiology::Medical microbiology
Handle http://hdl.handle.net/2043/11160 (link to this page)

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