Effects of clinical isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation

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Effects of clinical isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation

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Publication Article, peer reviewed scientific
Title Effects of clinical isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation
Author(s) Pihl, Maria ; Chávez de Paz, Luis Eduardo ; Schmidtchen, Artur ; Svensäter, Gunnel ; Davies, Julia
Date 2010
English abstract
Pseudomonas aeruginosa is often found in chronic infections, including cystic fibrosis lung infections and those related to chronic wounds and venous ulcers. At the latter sites, P. aeruginosa can be isolated together with Staphylococcus epidermidis, and we have therefore explored the effect of clinical isolates and laboratory strains of P. aeruginosa strains on colonisation by S. epidermidis in dual-species biofilms. Biofilm formation was assayed using 16S rRNA fluorescence in situ hybridization and confocal laser scanning microscopy. Amongst the six P. aeruginosa strains tested, one particular strain, denoted 14:2, exerted a significant inhibitory effect and even after six hours, S. epidermidis levels in dual-species biofilms were reduced by more than 85% compared to biofilms without P. aeruginosa. Interestingly strain 14:2 was found to be negative for classical virulence determinants including pyocyanin, elastase and alkaline protease. Therefore, we suggest that less virulent phenotypes of P. aeruginosa which may develop over time in chronic infections, could counteract colonisation by S. epidermidis, ensuring persistence and dominance by P. aeruginosa in the host micro-habitat. Further studies are required to explain the inhibitory effect on S. epidermidis, although, extracellular polysaccharides produced by P. aeruginosa might play a role in this phenomenon.
DOI http://dx.doi.org/10.1111/j.1574-695X.2010.00707.x (link to publisher's fulltext)
Publisher Wiley Blackwell
Host/Issue FEMS Immunology and Medical Microbiology;3
Volume 59
ISSN 0928-8244
Pages 504-512
Language eng (iso)
Subject(s) dual-species biofilms
Adhesion
extracellular products
strain differences
competition
Medicine
Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases
Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Microbiology::Medical microbiology
Handle http://hdl.handle.net/2043/11161 (link to this page)

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