N-terminal cleavage of the salivary MUC5B mucin. Analogy with the Van Willebrand propolypeptide?

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N-terminal cleavage of the salivary MUC5B mucin. Analogy with the Van Willebrand propolypeptide?

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Publication Article, peer reviewed scientific
Title N-terminal cleavage of the salivary MUC5B mucin. Analogy with the Van Willebrand propolypeptide?
Author Wickström, Claes ; Carlstedt, Ingemar
Date 2001
English abstract
Sequence similarities between the oligomeric mucins (MUC2, MUC5AC, MUC5B) and the von Willebrand factor suggest that they may be assembled in a similar way. After oligomerization, a fragment corresponding to the D1 and D2 domains is released from the von Willebrand factor. This cleavage does not appear to occur in pig submaxillary mucin, the only mammalian mucin in which this cleavage has been examined thus far, but whether other oligomeric mucins undergo N terminus proteolysis is not known. Antibodies recognizing the D1, D2, D3, and the first Cys domains in MUC5B were established and used to investigate to what extent proteolytic cleavage occurs within the N-terminal part of salivary MUC5B. The antibodies against the D1 and D2 domains identified a polypeptide corresponding in size to a MUC5B fragment generated by cleavage within the D' domain analogously with the von Willebrand factor propolypeptide. The antibodies did not recognize the main mucin population, suggesting that the major part of salivary MUC5B is subjected to this cleavage. An antibody recognizing the D3 domain was used to reveal a second cleavage site in the "soluble" but not in the "insoluble" MUC5B fraction: the first structural difference observed between soluble and insoluble salivary MUC5B. The identification of these cleavage events shows that the N-terminal sites for MUC5B oligomerization are present in the D3 domain and/or in domains located C-terminal to this part of the molecule.
DOI http://dx.doi.org/10.1074/jbc.M106593200 (link to publisher's fulltext)
Host/Issue Journal of Biological Chemistry;50
Volume 276
ISSN 0021-9258
Pages 47116-47121
Language eng (iso)
Subject MUC5B
mucin
gel-forming
cleavage
Medicine
Research Subject Categories::MEDICINE
Research Subject Categories::NATURAL SCIENCES
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/11164 Permalink to this page
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