Digital holographic microscopy – innovative and non-destructive analysis of living cells

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Digital holographic microscopy – innovative and non-destructive analysis of living cells

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Publication BookChapter
Title Digital holographic microscopy – innovative and non-destructive analysis of living cells
Author(s) El-Schich, Zahra ; Mölder, Anna ; Sebesta, Mikael ; Gisselsson, Lennart ; Alm, Kersti ; Gjörloff Wingren, Anette
Date 2010
Editor(s) Mendez-Vilas, A; Díaz, J
English abstract
Digital holography is a novel technique that has been developed recently to study living cells. The technique is an innovative, non-destructive method with possibilities to study living cells over time. We are investigating cell number, growth, viability and death of adherent cells using digital holography, which is a novel, label-free, imaging technique for biological applications. We have recently demonstrated that digital holography is highly comparable to the conventional manual cell counting method using a hemocytometer (Mölder et al., 2008). Digital holography is a method that gives us information about the refractive index of cells, which can change under different circumstances. The technique is cheap, fast and simple to use. The unique measurable parameters are the cell number, cell area, thickness and volume, which can be transformed to proliferation, migration, viability and cell death. The digital holographic images produced can provide both quantitative and qualitative phase information from a single hologram. Future applications can include real-time cell monitoring of various parameters of cells of different diseases in response to clinically relevant compounds.
Publisher FORMATEX RESEARCH CENTER
Host/Issue Microscopy: Science, Technology, Applications and Education
Series/Issue Microscopy Book Series;4
Pages 8
Language eng (iso)
Subject(s) digital holography
cell studies
cancer
viability
refractive index
Technology
Research Subject Categories::TECHNOLOGY
Handle http://hdl.handle.net/2043/11217 (link to this page)

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