FcgammaRIIB inhibits the development of atherosclerosis in low-density lipoprotein receptor-deficient mice

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FcgammaRIIB inhibits the development of atherosclerosis in low-density lipoprotein receptor-deficient mice

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Publication Article, peer reviewed scientific
Title FcgammaRIIB inhibits the development of atherosclerosis in low-density lipoprotein receptor-deficient mice
Author(s) Zhao, Ming ; Wigren, Maria ; Dunér, Pontus ; Kolbus, Daniel ; Olofsson, Katarina ; Björkbacka, Harry ; Nilsson, Jan ; Nordin Fredrikson, Gunilla
Date 2010
English abstract
The immune processes associated with atherogenesis have received considerable attention during recent years. IgG FcRs (FcgammaR) are involved in activating the immune system and in maintaining peripheral tolerance. However, the role of the inhibitory IgG receptor FcgammaRIIB in atherosclerosis has not been defined. Bone marrow cells from FcgammaRIIB-deficient mice and C57BL/6 control mice were transplanted to low-density lipoprotein receptor-deficient mice. Atherosclerosis was induced by feeding the recipient mice a high-fat diet for 8 wk and evaluated using Oil Red O staining of the descending aorta at sacrifice. The molecular mechanisms triggering atherosclerosis was studied by examining splenic B and T cells, as well as Th1 and Th2 immune responses using flow cytometry and ELISA. The atherosclerotic lesion area in the descending aorta was ~5-fold larger in mice lacking FcgammaRIIB than in control mice (2.75 +/- 2.57 versus 0.44 +/- 0.42%; p < 0.01). Moreover, the FcgammaRIIB deficiency resulted in an amplified splenocyte proliferative response to Con A stimulation (proliferation index 30.26 +/- 8.81 versus 2.96 +/- 0.81%, p < 0.0001) and an enhanced expression of MHC class II on the B cells (6.65 +/- 0.64 versus 2.33 +/- 0.25%; p < 0.001). In accordance, an enlarged amount of CD25-positive CD4 T cells was found in the spleen (42.74 +/- 4.05 versus 2.45 +/- 0.31%; p < 0.0001). The plasma Ab and cytokine pattern suggested increased Th1 and Th2 immune responses, respectively. These results show that FcgammaRIIB inhibits the development of atherosclerosis in mice. In addition, they indicate that absence of the inhibiting IgG receptor cause disease, depending on an imbalance of activating and inhibiting immune cells.
DOI http://dx.doi.org/10.4049/jimmunol.0902654 (link to publisher's fulltext)
Host/Issue Journal of Immunology;5
Volume 184
ISSN 1550-6606
Pages 2253-2260
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/11248 (link to this page)

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