LPS interactions with immobilized and soluble antimicrobial peptides

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LPS interactions with immobilized and soluble antimicrobial peptides

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Publication Article, peer reviewed scientific
Title LPS interactions with immobilized and soluble antimicrobial peptides
Author(s) Gustafsson, Anna ; Olin, Anders ; Ljunggren, Lennart
Date 2010
English abstract
A promising approach in sepsis therapy is the use of peptides truncated from serum- and membrane-proteins with binding domains for LPS: antimicrobial peptides (AMPs). AMPs can be useful in combination with conventional antibiotics to increase killing and neutralize LPS. Although many AMPs show a high specifi city towards bacterial membranes, they can also exhibit toxicity, i.e. non-specifi c membrane lysis, of mammalian cells such as erythrocytes and therefore, unsuitable as systemic drugs. A way to overcome this problem may be an extracorporeal therapy with immobilized peptides. This study will compare neutralization of LPS using different AMPs in solution and when immobilized on to solid phases. The peptides ability to neutralize LPS-induced cytokine release in whole blood will also be tested. The peptides are truncated derivates from the known AMPs LL-37, SC4, BPI, S3Δ and CEME. Two different methods were used to immobilize peptides, biomolecular interaction analysis, and Pierce SulfoLink Coupling Gel. To investigate LPS binding in solution the LAL test was used. After whole blood incubation with LPS and AMPs ELISA was used to measure TNF α , IL-1 β and IL-6 production. The results suggest that immobilization of antimicrobial peptides does not inhibit their capacity to neutralize LPS, although there are differences between the peptides tested. Thus, peptides derived from LL-37 and CEME were more effi cient both in LPS binding and neutralizing LPS-induced cytokine production
DOI http://dx.doi.org/10.3109/00365511003663622 (link to publisher's fulltext)
Publisher Informa healthcare
Host/Issue Scandinavian Journal of Clinical & Laboratory Investigation;3
Volume 70
ISSN 0036-5513
Pages 194-200
Language eng (iso)
Subject(s) Cationic peptides
ELISA
Limulus test
surface plasmon resonance
Medicine
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/11755 (link to this page)

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