In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

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In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

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Publication Article, peer reviewed scientific
Title In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin
Author(s) Bougas, Kostas ; Franke Stenport, Victoria ; Currie, Fredrik ; Wennerberg, Ann
Date 2011
English abstract
Objectives: The aim of the present study was to evaluate calcium phosphate precipitation and the amount of precipitated protein on three potentially bioactive surfaces when adding laminin in simulated body fluid. Material and Methods: Blasted titanium discs were prepared by three different techniques claimed to provide bioactivity: alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA). A blasted surface incubated in laminin-containing simulated body fuid served as a positive control (B) while a blasted surface incubated in non laminin-containing simulated body fuid served as a negative control (B-). The immersion time was 1 hour, 24 hours, 72 hours and 1 week. Surface topography was investigated by interferometry and morphology by Scanning Electron Microscopy (SEM). Analysis of the precipitated calcium and phosphorous was performed by Energy Dispersive X-ray Spectroscopy (EDX) and the adsorbed laminin was quantified by iodine (125I) labeling. Results: SEM demonstrated that all specimens except for the negative control were totally covered with calcium phosphate (CaP) after 1 week. EDX revealed that B- demonstrated lower sum of Ca and P levels compared to the other groups after 1 week. Iodine labeling demonstrated that laminin precipitated in a similar manner on the possibly bioactive surfaces as on the positive control surface. Conclusions: Our results indicate that laminin precipitates equally on all tested titanium surfaces and may function as a nucleation center thus locally elevating the calcium concentration. Nevertheless further studies are required to clarify the role of laminin in the interaction of biomaterials with the host bone tissue.
DOI http://dx.doi.org/10.5037/jomr.2011.2303 (link to publisher's fulltext)
Link http://www.ejomr.org/JOMR/archives/2011/3/e3/v2n3e3ht.htm (external link to publication)
Publisher STILUS OPTIMUS
Host/Issue Journal of Oral & Maxillofacial Research;3
Volume 2
Pages e3
Language eng (iso)
Subject(s) laminin
titanium
body fluid
calcium phosphates
biomaterials
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/12581 (link to this page)

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