Albumin-based nanoparticles as magnetic resonance contrast agents: I. Concept, first syntheses and characterisation

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Albumin-based nanoparticles as magnetic resonance contrast agents: I. Concept, first syntheses and characterisation

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Publication Article, peer reviewed scientific
Title Albumin-based nanoparticles as magnetic resonance contrast agents: I. Concept, first syntheses and characterisation
Author(s) Stollenwerk, Maria M ; Pashkunova-Martic, I ; Kremser, Christian ; Talasz, H ; Thurner, GC ; Abdelmoez, AA ; Wallnöfer, EA ; Helbok, A ; Neuhauser, E ; Klammsteiner, N ; Klimaschewski, L ; von Guggenberg, E ; Fröhlich, E ; Keppler, B ; Jaschke, W ; Debbage, Paul
Date 2010
English abstract
Abstract To develop a platform for molecular magnetic resonance imaging, we prepared gadolinium-bearing albumin-polylactic acid nanoparticles in the size range 20–40 nm diameter. Iterative cycles of design and testing upscaled the synthesis procedures to gram amounts for physicochemical characterisation and for pharmacokinetic testing. Morphological analyses showed that the nanoparticles were spheroidal with rough surfaces. Particle sizes were measured by direct transmission electron microscopical measurements from negatively contrasted preparations, and by use of photon correlation spectroscopy; the two methods each documented nanoparticle sizes less than 100 nm and generally 10–40 nm diameter, though with significant intrabatch and interbatch variability. The particles’ charge sufficed to hold them in suspension. HSA retained its tertiary structure in the particles. The nanoparticles were stable against turbulent flow conditions and against heat, though not against detergents. MRI imaging of liquid columns was possible at nanoparticle concentrations below 10 mg/ml. The particles were non-cytotoxic, non-thrombogenic and non-immunogenic in a range of assay systems developed for toxicity testing of nanoparticles. They were micellar prior to lyophilisation, but loosely structured aggregated masses after lyophilisation and subsequent resuspension. These nanoparticles provide a platform for further development, based on non-toxic materials of low immunogenicity already in clinical use,not expensive, and synthesized using methods which can be upscaled for industrial production.
DOI http://dx.doi.org/10.1007/s00418-010-0676-z (link to publisher's fulltext)
Publisher Springer
Host/Issue Histochemistry and Cell Biology;4
Volume 133
ISSN 0948-6143
Pages 375-404
Language eng (iso)
Subject(s) Human serum albumin
nanoparticles
MRI
contrast medium
chelate
gadolinium
Sciences
Research Subject Categories::INTERDISCIPLINARY RESEARCH AREAS
Handle http://hdl.handle.net/2043/13127 (link to this page)

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