Very low-density lipoprotein induces interleukin-1beta expression in macrophages.

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Very low-density lipoprotein induces interleukin-1beta expression in macrophages.

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Publication Article, peer reviewed scientific
Title Very low-density lipoprotein induces interleukin-1beta expression in macrophages.
Author(s) Stollenwerk, Maria M ; Lindholm, Marie ; Pörn-Ares, Isabella ; Larsson, Anna ; Nilsson, Jan ; Ares, Mikko PS
Date 2005
English abstract
Elevated plasma level of very low-density lipoprotein (VLDL) is a risk factor for coronary heart disease. We investigated the effect of VLDL on expression of the pro-inflammatory cytokine interleukin-1beta (IL-1beta) in human peripheral blood monocyte-derived macrophages. IL-1beta mRNA and protein expression was analysed by PCR and ELISA, respectively. Caspase activation was assessed by immunoblotting. Apart from potentiating lipopolysaccharide-induced secretion of IL-1beta, VLDL alone induced secretion of IL-1beta from human monocyte-derived macrophages. This effect was suppressed by an inhibitor of caspase-1, the protease which cleaves pro-IL-1beta. VLDL treatment activated caspase-1, as indicated by increased levels of the caspase-1 p20 subunit. Furthermore, VLDL increased IL-1beta mRNA expression, which was associated with activation of transcription factor AP-1. Inhibition of caspase-1 did not influence IL-1beta mRNA expression. In conclusion, VLDL induces IL-1beta mRNA expression, caspase-1 activation, and IL-1beta release from macrophages, suggesting that VLDL can promote inflammation in atherosclerotic lesions.
DOI http://dx.doi.org/10.1016/j.bbrc.2005.07.123 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Biochemical and biophysical research communications;2
Volume 335
ISSN 0006-291X
Pages 603-608
Language eng (iso)
Subject(s) VLDL
Interleukin-1beta
inflammation
macrophages
Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/13330 (link to this page)

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