Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

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Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

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Publication Article, peer reviewed scientific
Title Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts
Author Schwartz Filho, Humberto Osvaldo ; Morandini, Ana Carolina Faria ; Ramos-Junior, Erivan Schnaider ; Jimbo, Ryo ; Santos, Carlos Ferreira ; Marcantonio, Elcio Jr ; Wennerberg, Ann ; Marcantonio, Rosemary Adriana Chiérici
Date 2012
English abstract
Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-β), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). On one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). On the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and ON surfaces, but not with BAE+ON. Additionally, TGF-β and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs.
DOI http://dx.doi.org/10.1002/jbm.a.34200 (link to publisher's fulltext)
Publisher Wiley
Host/Issue Journal of Biomedical Materials Research A;10
Volume 100
ISSN 1549-3296
Pages 2629-2636
Language eng (iso)
Subject Wiley
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/14692 Permalink to this page
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