The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation

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The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation

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Publication Article, peer reviewed scientific
Title The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation
Author(s) Schwartz Filho, Humberto Osvaldo ; Bougas, Kostas ; Coelho, Paulo G ; Xue, Ying ; Hayashi, Mariko ; Faeda, Rafael Silveira ; Marcantonio, Rosemary Adriana Chiérici ; Ono, Daisuke ; Kobayashi, Fumio ; Kamal, Mustafa ; Wennerberg, Ann ; Jimbo, Ryo
Date 2012
English abstract
Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.
DOI http://dx.doi.org/10.1155/2012/305638 (link to publisher's fulltext)
Publisher Hindawi
Host/Issue International Journal of Biomaterials;305638
Volume 2012
ISSN 1687-8787
Pages 9 p
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/14706 (link to this page)

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