In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO2 coated surfaces

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In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO2 coated surfaces

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Publication Article, peer reviewed scientific
Title In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO2 coated surfaces
Author(s) Hayashi, Mariko ; Jimbo, Ryo ; Lindh, Liselott ; Sotres, Javier ; Sawase, Takashi ; Mustafa, Kamal ; Andersson, Martin ; Wennerberg, Ann
Date 2012
English abstract
The aims of the study were to characterize a nanostructured photoactive titanium dioxide (TiO(2)) coating and to compare the cellular response of human osteoblasts before and after ultraviolet (UV) irradiation of the coating. A specific nanostructured TiO(2) powder (Degussa P-25), which consists of approximately 80% anatase and 20% rutile, was spin-coated onto commercially pure titanium discs, and was heat-treated thereafter. After topographical, chemical and photocatalytic property characterizations, human osteoblasts were cultured on the coated discs before and after UV irradiation. Cell morphology was evaluated by scanning electron microscopy (SEM), and cell viability was analysed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. From the contact angle analysis, the wettability significantly improved after UV irradiation. The cultured cells were flattened with numerous elongated lammellipodia; however, no morphological differences were indicated between -UV and +UV surfaces. The MTT assay analysis showed that -UV surface presented significantly higher viability compared to the +UV surface except for one cell population group at 3h where there were no differences. The nanostructured photoactive TiO(2) surface improved its hydrophilicity by UV irradiation, however no enhancing effect in cell response was confirmed at the time tested compared to the non-irradiated surface.
DOI http://dx.doi.org/10.1016/j.actbio.2012.03.010 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Acta Biomaterials;6
Volume 8
ISSN 1742-7061
Pages 2411-2416
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/14717 (link to this page)

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