Effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen

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Effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen

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Publication Article, peer reviewed scientific
Title Effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen
Author(s) Fransson, Helena ; Petersson, Kerstin ; Davies, Julia
Date 2014
English abstract
AIM: To study how products released from different bacteria in a deep carious lesion affect the metabolic activity of odontoblast-like cells and their ability to produce the major organic component of dentine, collagen 1. METHODOLOGY: MDPC-23 cells were exposed to supernatants from biofilm cultures of strains isolated from the deepest part of a carious lesion as well as from a clinical isolate of Enterococcus faecalis. Lipoteichoic acid (LTA) and lipopolysaccharide (LPS) were used for comparison. Cell activity was assessed using an methyl-thiazolyl-diphenyl tetrazolium bromide (MTT) assay, and collagen 1 levels were determined by ELISA. RESULTS:The lesion microflora was dominated by Lactobacillus spp. Neither extracellular products from the isolates nor LPS affected the activity of the MDPC-23 cells, whereas extracellular products from E. faecalis and LTA significantly reduced total cell activity (P < 0.01). Enterococcus faecalis had an inhibitory effect upon collagen 1 production by the cells, whereas no such effect or even a slight stimulatory effect was seen for the isolates from the deep carious lesion. CONCLUSIONS: These studies indicate that culture supernatants from E. faecalis reduced the metabolic activity of odontoblast-like cells as shown using the MTT assay. No effect was seen for supernatants from biofilms of bacteria recovered from a deep carious lesion. Different bacteria varied in their effects upon collagen 1 production suggesting that the nature of the bacterial species in a carious lesion may have a direct influence upon the ability of the odontoblasts to produce tertiary dentine.
DOI http://dx.doi.org/10.1111/iej.12160 (link to publisher's fulltext)
Publisher Wiley
Host/Issue International Endodontic Journal;4
Volume 47
ISSN 1365-2591
Pages 397–404
Language eng (iso)
Subject(s) bacteria
biofilms
collagen type 1
dental caries
dental pulp
odontoblasts
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/15892 (link to this page)

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