LPS induces GROα chemokine production via NFκB in oral fibroblasts

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LPS induces GROα chemokine production via NFκB in oral fibroblasts

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Publication Article, peer reviewed scientific
Title LPS induces GROα chemokine production via NFκB in oral fibroblasts
Author(s) Jönsson, Daniel ; Amisten, Stefan ; Bratthall, Gunilla ; Holm, Anders ; Nilsson, Bengt-Olof
Date 2009
English abstract
OBJECTIVE AND DESIGN: Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROalpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROalpha by oral fibroblasts have not previously been presented. MATERIALS AND METHODS: GROalpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. RESULTS: We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROalpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROalpha and GROalpha protein. The steroid hormone estrogen had no effect on LPS-induced GROalpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROalpha production, and the NF-kappaB blocker MG 132 fully prevented LPS-induced GROalpha. CONCLUSIONS: Oral fibroblasts respond to LPS stimulation by increasing GROalpha production via the transcription factor NF-kappaB, suggesting that this mechanism may be involved in development of periodontal inflammation.
DOI http://dx.doi.org/10.1007/s00011-009-0049-z (link to publisher's fulltext)
Publisher Springer
Host/Issue Inflammation Research;58
Volume 11
ISSN 1023-3830
Pages 791-796
Language eng (iso)
Subject(s) Chemokines
Dexamethasone
Estrogen
GROα
PDL cells
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/15976 (link to this page)

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