Cells and holograms : holograms and digital holographic microscopy as a tool to study the morphology of living cells

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Cells and holograms : holograms and digital holographic microscopy as a tool to study the morphology of living cells

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Publication BookChapter
Title Cells and holograms : holograms and digital holographic microscopy as a tool to study the morphology of living cells
Author(s) Alm, Kersti ; El-Schich, Zahra ; Falck Miniotis, Maria ; Gjörloff Wingren, Anette ; Janicke, Birgit ; Oredsson, Stina
Date 2013
Editor(s) Mihaylova, Emilia
English abstract
We present a method to study the morphology of living, dividing and dying cells using DHM. DHM is a non-invasive, non-destructive and non-phototoxic method which allows the user to perform both qualitative and quantitative measurements of living cells over time. We show here our results on cell division and cell death in single cells. The morphological analyses performed here show changes caused by cell death and cell division, and indicate the possibilities to discriminate between different types of cell death. Cells dying in an apoptosis-like manner display different cell area and cell thickness profiles over time compared to cells dying in a necrosis-like manner, although their volume profiles are very similar. Dividing cells show a characteristic dip in the volume profile, which makes them easily distinguishable. Also, several previous studies show the versatile abilities of DHM. Different cell types have been studied and the morphology has been used to determine cell functionality as well as changes in morphology related to the environment. Cell morphology parameters can be very useful when following the effects of different treatments, the process of differentiation as well as cell growth and cell death. Cell morphology studied by DHM can be useful in toxicology, stem cell and cancer research.
DOI http://dx.doi.org/10.5772/54505 (link to publisher's fulltext)
Publisher Intech
Host/Issue Holography : basic principles and contemporary applications
ISBN 978-953-51-1117-7
Pages 335-351
Language eng (iso)
Subject(s) digital
holography
microscopy
cell division
cell death
noninvasive
morphology
eukaryotic
living cell
imaging
Technology
Research Subject Categories::TECHNOLOGY
Handle http://hdl.handle.net/2043/16146 (link to this page)

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