Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces

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Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces

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Publication Article, peer reviewed scientific
Title Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces
Author(s) Sotres, Javier ; Barrantes, Alejandro ; Lindh, Liselott ; Arnebrant, Thomas
Date 2014
English abstract
We show in this work how systems formed by phosphoproteins on calcium phosphate surfaces can be directly characterized, in real time, in liquid medium, without the need for elution or labeling. Specifically, we show how this is possible by applying three different techniques: ellipsometry, quartz crystal microbalance with dissipation, and atomic force microscopy-based friction force spectroscopy. We apply these techniques to study two different model systems, i.e. those formed upon the adsorption of two model phosphoproteins (κ- and β-casein) on hydroxyapatite (HA) surfaces. Information on the kinetics of adsorption, surface excess, viscoelasticity, water content, thickness of the layers, and protein-surface interaction is provided. Results indicate that both phosphoproteins form homogeneous elastic highly hydrated monolayers on the HA surfaces, the strength of β-casein layers being higher by approximately a factor of 4. Based on the experimental results, models for the conformation of κ- and β-casein molecules adsorbed on HA surfaces are proposed.
DOI http://dx.doi.org/10.1159/000351871 (link to publisher's fulltext)
Publisher Karger
Host/Issue Caries Research;2
Volume 48
ISSN 0008-6568
Pages 98-110
Language eng (iso)
Subject(s) Phosphoprotein
casein
hydroxyapatite
atomic force microscopy
friction force spectroscopy
ellipsometry
quartz crystal microbalance with dissipation
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/16248 (link to this page)

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