Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode

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Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode

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Publication Article, peer reviewed scientific
Title Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode
Author(s) Pita, Marcos ; Gutierrez-Sanchez, Cristina ; Toscano, Miguel ; Shleev, Sergey ; De Lacey, Antonio
Date 2013
English abstract
Gold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-​less biosensors for oxygen in a buffer that mimics the content and the compn. of human physiol. fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6 ± 1 μM with a linear range of 6-​300 μM, i.e. exceeding usual physiol. ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-​reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase.
DOI http://dx.doi.org/10.1016/j.bioelechem.2013.07.001 (link to publisher's fulltext)
Link http://www.sciencedirect.com/science/article/pii/S1567539413000595 (external link to publication)
Publisher Elsevier
Host/Issue Bioelectrochemistry;
Volume 94
ISSN 1567-5394
Pages 69-74
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/16369 (link to this page)

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