Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy

DSpace Repository

Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy

Show full item record

Files for download

Find Full text There are no files associated with this item.

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy
Author(s) Albèr, Cathrine ; Brandner, Birgit ; Björklund, Sebastian ; Billsten, Peter ; Corkery, Robert ; Engblom, Johan
Date 2013
English abstract
The rather thin outermost layer of the mammalian skin, stratum corneum (SC), is a complex biomembrane which separates the water rich inside of the body from the dry outside. The skin surface can be exposed to rather extreme variations in ambient conditions (e.g. water activity, temperature and pH), with potential effects on the barrier function. Increased understanding of how the barrier is affected by such changes is highly relevant for regulation of transdermal uptake of exogenous chemicals. In the present study we investigate the effect of hydration and the use of a well-known humectant, urea, on skin barrier ultrastructure by means of confocal Raman microspectroscopy. We also perform dynamic vapor sorption (DVS) microbalance measurements to examine the water uptake capacity of SC pretreated with urea. Based on novel Raman images, constructed from 20 spectral maps, we can distinguish large water inclusions within the skin membrane exceeding the size of fully hydrated corneocytes. We show that these inclusions contain water with spectral properties similar to that of bulk water. The results furthermore show that the ambient water activity has an important impact on the formation of these water inclusions as well as on the hydration profile across the membrane. Urea significantly increases the water uptake when present in skin, as compared to skin without urea, and it promotes formation of larger water inclusions in the tissue. The results confirm that urea can be used as a humectant to increase skin hydration.
DOI http://dx.doi.org/10.1016/j.bbamem.2013.06.011 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue BBA - Biomembranes;11
Volume 1828
ISSN 0005-2736
Pages 2470-2478
Language eng (iso)
Subject(s) Skin hydration
Water activity
Urea
Humectant
Confocal Raman microspectroscopy
Dynamic vapor sorption microbalance
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/16439 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics