Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique

DSpace Repository

Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique

Show full item record

Files for download

Find Full text There are no files associated with this item.

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique
Author(s) Graf, Gesche ; Kocherbitov, Vitaly
Date 2013
English abstract
The high-resolution humidity scanning QCM-D technique enables investigation of hydration of soft matter films using a quartz crystal microbalance with dissipation monitoring (QCM-D) equipped with a humidity module. Based on a continuous increase of relative humidity, properties of soft matter films can be investigated depending on the water content of the surrounding atmosphere. Determination of complete water sorption isotherms is possible via analysis of the overtone dependence of the resonance frequencies. Rheological properties are monitored via measurement of the dissipation. The glass transition can be identified from the change of viscoelastic properties of the film reflected in changes of the dissipation. A high-resolution water sorption isotherm of lysozyme was measured and compared with results from water sorption calorimetry. Analysis of the rheological behavior during hydration of lysozyme films revealed the presence of two separate sharp transitions at the water activities 0.67 and 0.91, which are connected to the glass transition. In previous works, only the existence of a broad glass transition has been reported so far. Combining the QCM-D data with Raman scattering data presented earlier, a new mechanism of isothermal glass transition in lysozyme is proposed.
DOI http://dx.doi.org/10.1021/jp404138f (link to publisher's fulltext)
Publisher American Chemical Society ACS
Host/Issue The Journal of Physical Chemistry B;34
Volume 117
ISSN 1520-6106
Pages 10017–10026
Language eng (iso)
Subject(s) Hydration
Lysozyme
Sorption Calorimetry
QCM-D
quartz crystal microbalance with dissipation monitoring
Humidity
water content
LiCl
Glass transition
Rheology
G'/G''
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/16490 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics