Blood tolerant laccase by directed evolution

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Blood tolerant laccase by directed evolution

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Publication Article, peer reviewed scientific
Title Blood tolerant laccase by directed evolution
Author(s) Mate, Diana ; Gonzalez-Perez, David ; Falk, Magnus ; Kittl, Roman ; Pita, Marcos ; De Lacey, Antonio ; Ludwig, Roland ; Shleev, Sergey ; Alcalde, Miguel
Date 2013
English abstract
High-​redox potential laccases are powerful biocatalysts with a wide range of applications in biotechnol. We have converted a thermostable laccase from a white-​rot fungus into a blood tolerant laccase. Adapting the fitness of this laccase to the specific compn. of human blood (above neutral pH, high chloride concn.) required several generations of directed evolution in a surrogate complex blood medium. Our evolved laccase was tested in both human plasma and blood, displaying catalytic activity while retaining a high redox potential at the T1 copper site. Mutations introduced in the second coordination sphere of the T1 site shifted the pH activity profile and drastically reduced the inhibitory effect of chloride. This proof of concept that laccases can be adapted to function in extreme conditions opens an array of opportunities for implantable nanobiodevices, chem. syntheses, and detoxification.
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Publisher Elsevier
Host/Issue Chemistry & Biology;2
Volume 20
ISSN 1074-5521
Pages 223-231
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
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