Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes

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Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes

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Publication Article, peer reviewed scientific
Title Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes
Author(s) Salaj-Kosla, Urszula ; Poller, Sascha ; Schuhmann, Wolfgang ; Shleev, Sergey ; Magner, Edmond
Date 2013
English abstract
The enzyme Trametes hirsuta laccase undergoes direct electron transfer at unmodified nanoporous gold electrodes, displaying a c.d. of 28 μA​/cm2. The response indicates that ThLc was immobilized at the surface of the nanopores in a manner which promoted direct electron transfer, in contrast to the absence of a response at unmodified polycryst. gold electrodes. The bioelectrocatalytic activity of ThLc modified nanoporous gold electrodes was strongly dependent on the presence of halide ions. Fluoride completely inhibited the enzymic response, whereas in the presence of 150 mM Cl-​, the current was reduced to 50​% of the response in the absence of Cl-​. The current increased by 40​% when the temp. was increased from 20 °C to 37 °C. The response is limited by enzymic and​/or enzyme electrode kinetics and is 30​% of that obsd. for ThLc co-​immobilized with an osmium redox polymer.
DOI http://dx.doi.org/10.1016/j.bioelechem.2012.11.001 (link to publisher's fulltext)
Link http://www.sciencedirect.com/science/article/pii/S1567539412001818 (external link to publication)
Publisher Elsevier
Host/Issue Bioelectrochemistry;
Volume 91
ISSN 1567-5394
Pages 15-20
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/16496 (link to this page)

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