Aluminium based adjuvants and their effects on mitochondria and lysosomes of phagocytosing cells

DSpace Repository

Aluminium based adjuvants and their effects on mitochondria and lysosomes of phagocytosing cells

Show full item record

Files for download

Find Full text There are no files associated with this item.

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title Aluminium based adjuvants and their effects on mitochondria and lysosomes of phagocytosing cells
Author(s) Ohlsson, Lars ; Exley, Christopher ; Darabi, Anna ; Sandén, Emma ; Siesjö, Peter ; Eriksson, Håkan
Date 2013
English abstract
Aluminium oxyhydroxide, Al(OH)3 is one of few compounds approved as an adjuvant in human vaccines. However, the mechanism behind its immune stimulating properties is still poorly understood. In vitro co- culture of an aluminium adjuvant and the human monocytic cell line THP-1 resulted in reduced cell proliferation. Inhibition occurred at concentrations of adjuvant several times lower than would be found at the injection site using a vaccine formulation containing an aluminium adjuvant. Based on evaluation of the mitochondrial membrane potential, THP-1 cells showed no mitochondrial rupture after co-culture with the aluminium adju- vant, instead an increase in mitochondrial activity was seen. The THP-1 cells are phagocytosing cells and after co-culture with the aluminium adjuvant the phagosomal pathway was obstructed. Primary or early phagosomes mature into phagolysosomes with an internal pH of 4.5 – 5 and carry a wide variety of hydrolysing enzymes. Co-culture with the aluminium adjuvant yielded a reduced level of acidic vesicles and cathepsin L activity, a proteolytic enzyme of the phagolysosomes, was almost completely inhibited. THP-1 cells are an appropriate in vitro model in order to investigate the mechanism behind the induction of a phagocytosing antigen presenting cell into an inflammatory cell by aluminium adjuvants. Much information will be gained by investigating the phagosomal pathway and what occurs inside the phagosomes and to elucidate the ultimate fate of phagocytosed aluminium particles.
DOI http://dx.doi.org/10.1016/j.jinorgbio.2013.08.003 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Journal of Inorganic Biochemistry;128
Volume 128 (2013)
ISSN 1873-3344
Pages 229-236
Language eng (iso)
Subject(s) Aluminium adjuvant
Inflammasome
NLRP3
Mitochondria and phagolysosome
Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/16508 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics