Alzheimer’s Disease Amyloid Peptides Interact with DNA, As Proved by Surface Plasmon Resonance

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Alzheimer’s Disease Amyloid Peptides Interact with DNA, As Proved by Surface Plasmon Resonance

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Publication Article, peer reviewed scientific
Title Alzheimer’s Disease Amyloid Peptides Interact with DNA, As Proved by Surface Plasmon Resonance
Author(s) Barrantes, Alejandro ; Camero, Sergio ; Garcia-Lucas, Angel ; Navarro, Pedro J. ; Benitez, Maria J. ; Jimenez, Juan S.
Date 2012
English abstract
According to the amyloid hypothesis, abnormal processing of the β-amyloid precursor protein in Alzheimer's disease patients increases the production of β-amyloid toxic peptides, which, after forming highly aggregated fibrillar structures, lead to extracellular plaques formation, neuronal loss and dementia. However, a great deal of evidence has point to intracellular small oligomers of amyloid peptides, probably transient intermediates in the process of fibrillar structures formation, as the most toxic species. In order to study the amyloid-DNA interaction, we have selected here three different forms of the amyloid peptide: Aβ1-40, Aβ25-35 and a scrambled form of Aβ25-35. Surface Plasmon Resonance was used together with UV-visible spectroscopy, Electrophoresis and Electronic Microscopy to carry out this study. Our results prove that, similarly to the full length Aβ1-42, all conformations of toxic amyloid peptides, Aβ1-40 and Aβ25-35, may bind DNA. In contrast, the scrambled form of Aβ25-35, a non-aggregating and nontoxic form of this peptide, could not bind DNA. We conclude that although the amyloid-DNA interaction is closely related to the amyloid aggregation proneness, this cannot be the only factor which determines the interaction, since small oligomers of amyloid peptides may also bind DNA if their predominant negatively charged amino acid residues are previously neutralized.
DOI http://dx.doi.org/10.2174/156720512803251101 (link to publisher's fulltext)
Publisher Bentham
Host/Issue Current Alzheimer Research;8
Volume 9
ISSN 1567-2050
Pages 924-934
Language eng (iso)
Subject(s) Alzheimer
amyloid
DNA
surface plasmon resonance
Electron Microscopy
peptide
UV spectrum
Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/17737 (link to this page)

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