Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells

DSpace Repository

Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells

Show full item record

Files for download

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells
Author(s) Falck Miniotis, Maria ; Mukwaya, Anthonny ; Gjörloff Wingren, Anette
Date 2014
English abstract
Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells.
DOI http://dx.doi.org/10.1371/journal.pone.0106546 (link to publisher's fulltext)
Publisher Public Library of Science
Host/Issue PLoS one;1
Volume 9
ISSN 1932-6203
Pages 1-6
Language eng (iso)
Subject(s) Cancer treatment
Digital holographic microscopy
Cell cycle
Medicine
Research Subject Categories::INTERDISCIPLINARY RESEARCH AREAS
Handle http://hdl.handle.net/2043/17784 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics