Formation of supported lipid bilayers by vesicle fusion : effect of deposition temperature

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Formation of supported lipid bilayers by vesicle fusion : effect of deposition temperature

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Publication Article, peer reviewed scientific
Title Formation of supported lipid bilayers by vesicle fusion : effect of deposition temperature
Author(s) Lind, Tania ; Cárdenas, Marité ; Wacklin, Hanna
Date 2014
English abstract
We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (Tm) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above Tm by neutron reflection and investigated the effect of subsequent cooling to below the Tm. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid Tm. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.
DOI http://dx.doi.org/10.1021/la500897x (link to publisher's fulltext)
Publisher ACS
Host/Issue Langmuir;25
Volume 30
ISSN 0743-7463
Pages 7259-7263
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/17830 (link to this page)

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