Reproducibility of subgingival bacterial samples from patients with peri-implant mucositis

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Reproducibility of subgingival bacterial samples from patients with peri-implant mucositis

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Publication Article, peer reviewed scientific
Title Reproducibility of subgingival bacterial samples from patients with peri-implant mucositis
Author(s) Hallström, Hadar ; G Rutger, Persson ; Strömberg, Ulf ; Twetman, Svante ; Renvert, Stefan
Date 2014
English abstract
OBJECTIVE: The aim of the present study was to investigate the reproducibility of bacterial enumeration from subsequent subgingival samples collected from patients with peri-implant mucositis. MATERIAL AND METHODS: Duplicate microbial samples from 222 unique implant sites in 45 adult subjects were collected with paper points and analyzed using the checkerboard DNA-DNA hybridization technique. Whole genomic probes of 74 preselected bacterial species were used. Based on the bacterial scores, Cohen's kappa coefficient was used to calculate the inter-annotator agreement for categorical data. The percentage agreement was considered as "good" when the two samples showed the same score or differed by 1 to the power of 10. RESULTS: Moderate to fair kappa values were displayed for all bacterial species in the test panel (range 0.21-0.58). There were no significant differences between Gram-positive and Gram-negative species. The percentage of good agreement between the first and second samples averaged 74.7 % (n = 74; range 56-83 %), while the proportion of poor agreement ranged from 1 to 19 % for the various strains. CONCLUSION: While an acceptable clinical agreement was obtained in most cases, diverging bacterial scores may appear in subgingival samples collected at the same time point from patients with peri-implant mucositis. CLINICAL RELEVANCE: The broad bulky base of implant crowns may present an obstacle for the collection of reproducible subgingival samples with paper points.
DOI http://dx.doi.org/10.1007/s00784-014-1324-0 (link to publisher's fulltext)
Publisher Springer
Host/Issue Clinical Oral Investigations;
ISSN 1432-6981
Pages Epub ahead of print
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/17977 (link to this page)

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