Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase

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Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase

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Publication Article, peer reviewed scientific
Title Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase
Author Krikstolaityte, Vida ; Barrantes, Alejandro ; Ramanavicius, Arunas ; Arnebrant, Thomas ; Shleev, Sergey ; Ruzgas, Tautgirdas
Date 2014
English abstract
To characterise bioelectrocatalytic oxygen reduction at gold nanoparticles (AuNPs) modified with Trametes hirsuta laccase (ThLc) combined electrochemical and quartz crystal microbalance measurements have been used. The electrodes with different degrees of AuNP-monolayer coverage, theta, have been studied. In every case of theta close to theoretically possible 44 ThLc molecules adsorbed at 22 nm diameter AuNP. The bioelectrocatalytic current was recalculated down to the current at a single AuNR Unexpectedly, the current at a single AuNP was higher when theta was higher. The maximum current reached at a single AuNP was 31.10(-18) A which corresponds to the enzyme turnover (k(cat)) 13 s(-1). This rate is lower than the homogeneous ThLc turnover (190 s(-1)) suggesting partial denaturation of ThLc upon adsorption or that some ThLc are not in DET contact with the electrode surface
DOI http://dx.doi.org/10.1016/j.bioelechem.2013.09.004 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Bioelectrochemistry;
Volume 95
ISSN 1567-5394
Pages 1-6
Language eng (iso)
Subject Gold nanoparticle
Laccase
Direct electron transfer
Glucose/oxygen biofuel cell
Cellobiose dehydrogenase
Redox enzymes
Medicine
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/18093 Permalink to this page
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