Mediatorless Carbohydrate​/Oxygen Biofuel Cells with Improved Cellobiose Dehydrogenase Based Bioanode

DSpace Repository

Mediatorless Carbohydrate​/Oxygen Biofuel Cells with Improved Cellobiose Dehydrogenase Based Bioanode

Show full item record

Files for download

Find Full text There are no files associated with this item.

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title Mediatorless Carbohydrate​/Oxygen Biofuel Cells with Improved Cellobiose Dehydrogenase Based Bioanode
Author(s) Krikstolaityte, Vida ; Lamberg, Peter ; Toscano, Miguel ; Silow, Maria ; Eicher-Lorka, Olegas ; Ramanavicius, Arunas ; Niaura, Gediminias ; Abairute, Laura ; Ruzgas, Tautgirdas ; Shlee, Sergey
Date 2014
English abstract
Direct electron transfer (DET) between cellobiose dehydrogenase from Humicola insolens ascomycete (HiCDH) and gold nanoparticles (AuNPs) was achieved by modifying AuNPs with a novel, pos. charged thiol N-​(6-​mercapto)​hexylpyridinium (MHP)​. The DET enabled the use of the HiCDH enzyme as an anodic biocatalyst in the design of a mediatorless carbohydrate​/oxygen enzymic fuel cell (EFC)​. A biocathode of the EFC was based on bilirubin oxidase from Myrothecium verrucaria (MvBOx) directly immobilized on the surface of AuNPs. The following parameters of the EFC based on Au​/AuNP​/MHP​/HiCDH bioanode and Au​/AuNP​/MvBOx biocathode were obtained in quiescent air satd. PBS, pH 7.4, contg.: (i) 5 mM glucose-​open-​circuit voltage (OCV) of 0.65 ± 0.011 V and the maximal power d. of 4.77 ± 1.34 μW cm-​2 at operating voltage of 0.50 V; or (ii) 10 mM lactose-​OCV of 0.67 ± 0.006 V and the maximal power d. of 8.64 ± 1.91 μW cm-​2 at operating voltage of 0.50 V. The half-​life operation times of the EFC were estd. to be at least 13 and 44 h in air satd. PBS contg. 5 mM glucose and 10 mM lactose, resp. Among advantages of HiCDH​/MvBOx FCs are (i) simplified construction, (ii) relatively high power output with glucose as biofuel, and (iii) the absence of the inhibition of the HiCDH based bioanode by lactose, when compared with the best previously reported CDH based bioanode.
DOI http://dx.doi.org/10.1002/fuce.201400003 (link to publisher's fulltext)
Publisher Wiley
Host/Issue Fuel Cells;6
Volume 14
ISSN 1615-6854
Pages 792-800
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/18165 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics