Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces

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Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces

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Publication Article, peer reviewed scientific
Title Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces
Author(s) Kapp, Sebastian ; Larsson, Iben ; van de Weert, Marco ; Cárdenas, Marité ; Jørgensen, Lene
Date 2015
English abstract
Two monoclonal antibodies from the IgG subclasses one and two were compared in their adsorption behavior with hydrophobic surfaces upon dilution to 10 mg/mL with 0.9% NaCl. These conditions simulate handling of the compounds at hospital pharmacies and surfaces encountered after preparation, such as infusion bags and i.v. lines. Total internal reflection fluorescence and quartz crystal microbalance with dissipation monitoring were used to follow and quantify this. Furthermore, the influence of the nonionic surfactant polysorbate 80 (PS80) on the adsorption process of these two antibodies was investigated. Despite belonging to two different IgG subclasses, both antibodies displayed comparable adsorption behavior. Both antibodies readily adsorbed in the absence of PS80, whereas adsorption was reduced in the presence of 30 mg/L surfactant. The sequence of exposure of the surfactant and protein to the surface was found to have a major influence on the extent of protein adsorption. Although only a fraction of adsorbed protein could be removed by rinsing with 30 mg/L surfactant solution, adsorption was entirely prevented when surfaces were pre-exposed to PS80
DOI http://dx.doi.org/10.1002/jps.24265 (link to publisher's fulltext)
Publisher Wiley
Host/Issue Journal of Pharmaceutical Sciences;2
Volume 104
ISSN 1520-6017
Pages 593–601
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::PHARMACY
Handle http://hdl.handle.net/2043/18185 (link to this page)

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