A QCM-D Study of Reduced Antibody Fragments Immobilized on Planar Gold and Gold Nanoparticle Modified Sensor Surfaces

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A QCM-D Study of Reduced Antibody Fragments Immobilized on Planar Gold and Gold Nanoparticle Modified Sensor Surfaces

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Publication Article, peer reviewed scientific
Title A QCM-D Study of Reduced Antibody Fragments Immobilized on Planar Gold and Gold Nanoparticle Modified Sensor Surfaces
Author(s) Makaraviciute, Asta ; Ruzgas, Tautgirdas ; Ramanavicius, Arunas ; Ramanaviciene, Almira
Date 2014
English abstract
An immunosensor is an analytical system consisting of specific immune system molecules coupled to a signal transducer. Immunosensor sensitivity depends on the type of immunorecognition ligands used, immobilization influence on their activity and orientation on the surface. Quartz crystal microbalance with dissipation (QCM-D) was employed to investigate the immobilization of antibodies against bovine leukemia virus antigen gp51 (gp51). Disulphide bridges of antibody hinge region were reduced chemically to yield two “half” antibody fragments (Frag-Ab), each having a single antigen binding site and free sulfhydryl groups that were used for immobilization. Frag-Ab were immobilized on planar gold and gold nanoparticle (AuNP) modified QCM-D sensor surfaces from initial solutions of different concentrations. Higher Frag-Ab surface density values were obtained on AuNP modified surfaces at all tested antibody concentrations. Frag-Ab/gp51 specific interaction was registered and it was determined that the highest sensitivity was exhibited by Frag-Ab immobilized at the lowest surface desities on both types of investigated surfaces. Specific gp51 interaction with Frag-Ab and non-specific binding to bovine serum albumin modified surfaces were
DOI http://dx.doi.org/10.4028/www.scientific.net/KEM.605.340 (link to publisher's fulltext)
Publisher Trans Tech Publications
Host/Issue Key Engineering Materials;
Volume 605
ISSN 1662-9795
Pages 340-343
Language eng (iso)
Subject(s) immunosensor
biosensor
QCM-D
antibody
antibody fragments
gold nanoparticles
Medicine
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/18355 (link to this page)

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