Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line

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Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line

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Publication Article, peer reviewed scientific
Title Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line
Author(s) Mold, Matt ; Eriksson, Håkan ; Siesjö, Peter ; Darabi, Anna ; Shardlow, Emma ; Exley, Christopher
Date 2014
English abstract
Aluminium-based adjuvants (ABA) are the predominant adjuvants used in human vaccinations. While a consensus is yet to be reached on the aetiology of the biological activities of ABA several studies have identified shape, crystallinity and size as critical factors affecting their adjuvanticity. In spite of recent advances, the fate of ABA following their administration remains unclear. Few if any studies have demonstrated the unequivocal presence of intracellular ABA. Herein we demonstrate for the first time the unequivocal identification of ABA within a monocytic (THP-1) cell line, using lumogallion as a fluorescent molecular probe for aluminium. Use of these new methods revealed that particulate ABA was only found in the cell cytoplasm. Transmission electron microscopy revealed that ABA were contained within vesicle-like structures of approximately 0.5–1 μm in diameter.
DOI http://dx.doi.org/10.1038/srep06287 (link to publisher's fulltext)
Publisher Nature
Host/Issue Scientific Reports;4
ISSN 2045-2322
Pages 6287
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/18360 (link to this page)

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