Antibody-free biomarker determination : exploring molecularly imprinted polymers for pro-gastrin releasing Peptide

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Antibody-free biomarker determination : exploring molecularly imprinted polymers for pro-gastrin releasing Peptide

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Publication Article, peer reviewed scientific
Title Antibody-free biomarker determination : exploring molecularly imprinted polymers for pro-gastrin releasing Peptide
Author Rossetti, Cecilia ; Qader, Abed Abdel ; Halvorsen, Trine Grönhaug ; Sellergren, Börje ; Reubsaet, Leon
Date 2014
English abstract
Biomarker mass spectrometry-assays are highly demanded and analysis of Pro-Gastrin Releasing Peptide (ProGRP) as Small Cell Lung Cancer marker has been recently investigated by mass spectrometry after immuno-extraction. In this paper we introduce an assay based on molecularly imprinted polymers (MIPs) targeting the proteotypic peptide of ProGRP, as a possible alternative to current immuno-based assay. The MIPs were prepared by surface initiated Reversible Addition-Fragmentation chain Transfer (RAFT) polymerization and were introduced as sorbents for clean-up and enrichment of ProGRP signature peptide from tryptically treated serum samples. The use of an appropriate solid phase extraction (SPE) protocol allowed specific extraction of the target peptide while depleting other peptides risen from the sample digestion, hence resulting in a reduced background. The selective extraction of ProGRP signature peptide, after serum samples digestion, translates into a time and cost-effective method suited for bottom-up analysis wherever targeted peptide extraction from complex matrices is required
DOI https://doi.org/10.1021/ac503559c (link to publisher's fulltext.)
Publisher American Chemical Society
Host/Issue Analytical Chemistry;24
Volume 86
ISSN 0003-2700
Pages 12291–12298
Language eng (iso)
Subject Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/18387 Permalink to this page
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