Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide

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Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide

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Publication Article, peer reviewed scientific
Title Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide
Author(s) Qader, Abed Abdel ; Urraca, Javier ; Torsetnes, Silje Böen ; Tönnesen, Finn ; Reubsaet, Leon ; Sellergren, Börje
Date 2014
English abstract
Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests.
DOI http://dx.doi.org/10.1016/j.chroma.2014.10.023 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Journal of chromatography. A.;
Volume 1370
ISSN 0021-9673
Pages 56-62
Language eng (iso)
Subject(s) Humanities/Social Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/18388 (link to this page)

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