Robust and selective nano cavities for protein separation : An interpenetrating polymer network modified hierarchically protein imprinted hydrogel

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Robust and selective nano cavities for protein separation : An interpenetrating polymer network modified hierarchically protein imprinted hydrogel

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Publication Article, peer reviewed scientific
Title Robust and selective nano cavities for protein separation : An interpenetrating polymer network modified hierarchically protein imprinted hydrogel
Author(s) Nematollahzadeh, Ali ; Lindemann, Patrik ; Sun, Wei ; Stute, Jörg ; Lütkemeyer, Dirk ; Sellergren, Börje
Date 2014
English abstract
In the present work, we report a novel method for the reinforcement of hierarchically structured molecularly imprinted polymer (MIP) for the separation of human serum albumin (HSA) and immunoglobulin G (IgG) proteins under pressure driven flow conditions. The template proteins (HSA or IgG) were first physically adsorbed at their isoelectric point on the surface of wide pore silica particles. Thereafter, the pore system was filled with a monomer solution and polymerized to form a lightly crosslinked polyacrylamide network covering the protein template. In order to enhance the rigidity of the hydrogels, different type of crosslinkers such as ethylene glycol dimethacrylate (EGDMA), 3,4-dihydroxyphenethylamine (DA) and methylene-bis-acrylamide (MBA), at least partially interpenetrated into the initially made acrylamide base hydrogel leading to formation of an interpenetrating polymer network (IPN). Then the silica matrix was removed to leave highly porous and reinforced MIP. TGA together with FT-IR and TEM analysis supported the interpenetration of the secondary crosslinkers in the initially formed polymer matrix. The compression property of the modified hydrogels as a function of degree of swelling was in the following order: DA>EGDMA>MBA-IPN-modified hydrogel. Batch binding assay verified the capability of the IPN modified MIPs to capture the target proteins. Moreover, solid phase extraction, HPLC and SDS-PAGE revealed that the EGDMA modified MIP could selectively capture and separate the proteins from human serum or fermentation broth
DOI http://dx.doi.org/10.1016/j.chroma.2014.04.030 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Journal of Chromatography A;
Volume 1345
ISSN 0021-9673
Pages 154-163
Language eng (iso)
Subject(s) Interpenetrating polymer network
Mechanical strength
Molecularly imprinted polymer
Protein separation
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/18391 (link to this page)

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