In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating

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In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating

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Publication Article, peer reviewed scientific
Title In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating
Author(s) Cecchinato, Francesca ; Xue, Ying ; Karlsson, Johan ; He, Wenxiao ; Wennerberg, Ann ; Mustafa, Kamal ; Andersson, Martin ; Jimbo, Ryo
Date 2013
English abstract
This work aimed to evaluate the in vitro response of Transfected Human Foetal Osteoblast (hFOB) cultured on a magnesium-loaded mesoporous TiO2 coating. The application of mesoporous films on titanium implant surfaces has shown very promising potential to enhance osseointegration. This type of coating has the ability to act as a framework to sustain bioactive agents and different drugs. Magnesium is the element that, after calcium, is the most frequently used to dope titanium implant surfaces, since it is crucial for protein formation, growth factor expression, and aids for bone mineral deposition on implant surfaces. Mesoporous TiO2 films with an average pore-size of 6 nm were produced by the evaporation-induced self-assembly method (EISA) and deposited onto titanium discs. Magnesium loading was performed by soaking the mesoporous TiO2discs in a magnesium chloride solution. Surface characterization was con- ducted by SEM, XPS, optical interferometry, and AFM. Magnesium release profile was assessed at different time points using a Magnesium Detection kit. Cell morphology and spreading were observed with SEM. The cytoskeletal organization was stained with TRITC-conjugated Phalloidin and cell viability was evaluated through a mitochondrial colorimetric (MTT) assay. Furthermore, gene expression of bone markers and cell mineralization were analyzed by real time RT-PCR and alizarin-red staining, respectively. The surface chemical analysis by XPS revealed the successful adsorption of magnesium to the mesoporous coating. The AFM measurements revealed the presence of a nanostructured surface roughness. Osteoblasts viability and adhesion as well as the gene expression were unaffected by the addition of magnesium possibly due to its rapid burst release, however, were enhanced by the 3D nanostructure of the TiO2l ayer.
DOI http://dx.doi.org/10.1002/jbm.a.35062 (link to publisher's fulltext)
Publisher Wiley
Host/Issue Journal of biomedical materials research. Part A;11
Volume 102
ISSN 1549-3296
Pages 3862-71
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/18426 (link to this page)

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