Caries correlates strongly with salivary levels of Matrix Metalloproteinase-8

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Caries correlates strongly with salivary levels of Matrix Metalloproteinase-8

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Publication Article, peer reviewed scientific
Title Caries correlates strongly with salivary levels of Matrix Metalloproteinase-8
Author(s) Hedenbjörk-Lager, Anders ; Bjørndal, Lars ; Gustafsson, Anders ; Sorsa, Timo ; Tjäderhane, Leo ; Åkerman, Sigvard ; Ericson, Dan
Date 2015
English abstract
The caries process in dentin involves the degradation of both mineral and organic matrix. The demineralization has been demonstrated to be caused by bacterial acids. How-ever, the collagen degradation is considered to be initiated by endogenous proteolytic enzymes, mainly collagenolytic matrix metalloproteinases (MMPs). This paper aims to relate salivary MMP-8 (or salivary collagenase-2) and tissue inhibi-tor of MMP (TIMP-1) levels to manifest caries in a large num-ber of subjects. A random sample of 451 adults (aged 18-87 years) living in the south of Sweden was included in this study. Standard clinical examinations were performed, and stimulated saliva was collected and analyzed for concentra-tions of MMP-8, TIMP-1 and total protein, using an immuno-fluorometric assay, an enzyme-linked immunosorbent assay and the Bradford assay, respectively. Salivary numbers of mutans streptococci and lactobacilli were determined using a chair-side kit. Subjects with manifest caries lesions present-ed with elevated levels of MMP-8 (p < 0.001) as well as total protein, MMP-8/TIMP-1 ratio, bleeding on probing and plaque index (p = 0.05) compared with subjects without manifest caries. Multiple linear regression analysis with car-ies as the dependent variable revealed MMP-8 as the only significant explanatory variable (p < 0.001). TIMP-1 was not significant in any case. Using MMP-8 as the dependent vari-able revealed total protein concentration, caries lesions (p ≤ 0.001) and salivary secretion rate (p = 0.05) as explanatory variables. In conclusion, our data reveal that subjects with manifest caries lesions have elevated levels of salivary MMP-8 relative to subjects with no caries lesions.
DOI (link to publisher's fulltext)
Publisher Karger
Host/Issue Caries Research;1
Volume 49
ISSN 0008-6568
Pages 1-8
Language eng (iso)
Subject(s) Dental caries
Matrix metalloproteinase-8
Tissue inhibitor of metalloproteinase-1
Research Subject Categories::ODONTOLOGY
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