Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume

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Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume

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Publication Article, peer reviewed scientific
Title Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume
Author El-Schich, Zahra ; Nilsson, Emmy ; Gerdtsson, Anna ; Wingren, Christer ; Gjörloff Wingren, Anette
Date 2015
English abstract
Background: We introduce the combination of digital holographic microscopy (DHM) and antibody microarrays as a powerful tool to measure morphological changes in specifically antibody-captured cells. The aim of the study was to develop DHM for analysis of cell death of etoposide-treated suspension cells. Result/Methodology: We demonstrate that the cell number, mean area, thickness, and volume were non-invasively measured by using DHM. The cell number was stable over time, but the two cell lines showed changes of cell area and cell irregularity after treatment. The cell volume in etoposide-treated cells was decreased, whereas untreated cells showed stable volume. Conclusions: Our results provide proof of concept for using DHM combined with antibody-based microarray technology for detecting morphological changes in captured cells.
Link http://www.future-science.com/doi/abs/10.4155/fso.14.2 (external link to publication)
Publisher Future Science Group
Host/Issue Future Science OA;
ISSN 2056-5623
Pages 11
Language eng (iso)
Subject antibody
cellular
holography
microarray
volume
Sciences
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/18923 Permalink to this page
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