Nanodisc films for membrane protein studies by neutron reflection : Effect of the protein scaffold choice

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Nanodisc films for membrane protein studies by neutron reflection : Effect of the protein scaffold choice

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Publication Article, peer reviewed scientific
Title Nanodisc films for membrane protein studies by neutron reflection : Effect of the protein scaffold choice
Author(s) Bertram, Nicolas ; Barker, Robert ; Bavishi, Krutika ; Lindberg Møller, Birger ; Cardenas, Marite
Date 2015
English abstract
Nanodisc films are a promising approach to study the equilibrium conformation of membrane bound proteins in native-like environment. Here we compare nanodisc formation for NADPH-dependent cytochrome P450 oxidoreductase (POR) using two different scaffold proteins, MSP1D1 and MSP1E3D1. Despite the increased stability of POR loaded MSP1E3D1 based nanodiscs in comparison to MSP1D1 based nanodiscs, neutron reflection at the silicon–solution interface showed that POR loaded MSP1E3D1 based nanodisc films had poor surface coverage. This was the case, even when incubation was carried out under conditions that typically gave high coverage for empty nanodiscs. The low surface coverage affects the embedded POR coverage in the nanodisc film and limits the structural information that can be extracted from membrane bound proteins within them. Thus, nanodisc reconstitution on the smaller scaffold proteins is necessary for structural studies of membrane bound proteins in nanodisc films.
DOI http://dx.doi.org/10.1021/acs.langmuir.5b00936 (link to publisher's fulltext)
Publisher American Chemical Society
Host/Issue Langmuir;30
Volume 31
ISSN 0743-7463
Pages 8386-8391
Language eng (iso)
Subject(s) Humanities/Social Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/19810 (link to this page)

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