Effect of fluoride and chlorhexidine digluconate mouthrinses on plaque biofilms

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Effect of fluoride and chlorhexidine digluconate mouthrinses on plaque biofilms

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Publication Article, peer reviewed scientific
Title Effect of fluoride and chlorhexidine digluconate mouthrinses on plaque biofilms
Author(s) Rabe, Per ; Twetman, Svante ; Kinnby, Bertil ; Svensäter, Gunnel ; Davies, Julia R
Date 2015
English abstract
Objective. To develop a model in which to investigate the architecture of plaque biofilms formed on enamel surfaces in vivo and to compare the effects of anti-microbial agents of relevance for caries on biofilm vitality. Materials and Methodology : Enamel discs mounted on healing abutments in the pre-molar region were worn by three subjects for 7 days. Control discs were removed before subjects rinsed with 0.1% chlorhexidine digluconate (CHX) or 0.2% sodium fluoride (NaF) for 1 minute. Biofilms were stained with Baclight Live/Dead and z-stacks of images created using confocal scanning laser micoscopy. The levels of vital and dead/damaged bacteria in the biofilms, assessed as the proportion of green and red pixels respectively, were analysed using ImageTrak(®) software. Results : The subjects showed individual differences in biofilm architecture. The thickness of the biofilms varied from 28-96µm although cell density was always the greatest in the middle layers. In control biofilms, the overall levels of vitality were high (71-98%) especially in the area closest to the enamel interface. Rinsing with either CHX or NaF caused a similar reduction in overall vitality. CHX exerted an effect throughout the biofilm, particularly on the surface of cell clusters whereas NaF caused cell damage/death mainly in the middle to lower biofilm layers. Conclusion : We describe a model that allows the formation of mature, undisturbed oral biofilms on human enamel surfaces in vivo and show that CHX and NaF have a similar effect on overall vitality but differ in their sites of action.
DOI http://dx.doi.org/10.2174/1874210601509010106 (link to publisher's fulltext)
Publisher Bentham Open
Host/Issue Open Dentistry Journal;
Volume 9
ISSN 1874-2106
Pages 106-111
Language eng (iso)
Subject(s) plaque
oral bacteria
oral cavity
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/19813 (link to this page)

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