Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies

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Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies

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Publication Article, peer reviewed scientific
Title Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies
Author(s) Chen, Jing ; Shinde, Sudhirkumar ; Koch, Markus Hermann ; Eisenacher, Martin ; Galozzi, Sara ; Lerari, Thilo ; Barkovits, Katalin ; Subedi, Prabal ; Krüger, Reiko ; Kuhlmann, Katja ; Sellergren, Börje ; Helling, Stefan ; Marcus, Katrin
Date 2015
English abstract
Phosphospecific enrichment techniques and mass spectrometry (MS) are essential tools for comprehending the cellular phosphoproteome. Here, we report a fast and simple approach for low sequence-bias phosphoserine (pS) peptide capture and enrichment that is compatible with low biological or clinical sample input. The approach exploits molecularly imprinted polymers (MIPs, "plastic antibodies") featuring tight neutral binding sites for pS or pY that are capable of cross-reacting with phosphopeptides of protein proteolytic digests. The versatility of the resulting method was demonstrated with small samples of whole-cell lysate from human embryonic kidney (HEK) 293T cells, human neuroblastoma SH-SY5Y cells, mouse brain or human cerebrospinal fluid (CSF). Following pre-fractionation of trypsinized proteins by strong cation exchange (SCX) chromatography, pS-MIP enrichment led to the identification of 924 phosphopeptides in the HEK 293T whole-cell lysate, exceeding the number identified by TiO2-based enrichment (230). Moreover, the phosphopeptides were extracted with low sequence bias and showed no evidence for the characteristic preference of TiO2 for acidic amino acids (aspartic and glutamic acid). Applying the method to human CSF led to the discovery of 47 phosphopeptides belonging to 24 proteins and revealed three previously unknown phosphorylation sites.
DOI http://dx.doi.org/10.1038/srep11438 (link to publisher's fulltext)
Publisher Nature Publishing Group
Host/Issue Scientific Reports;
Volume 5
ISSN 2045-2322
Pages 11438
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/19828 (link to this page)

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